Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody
Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB9577G, filled histogram) or unstained cells (open histogram).View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: EGFR
Cetuximab
is a biosimilar directed against the extracellular domain of EGFR. This
product can be used in flow cytometry or
various assay format to measure EGFR expression.
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Citation for Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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DNA damage alters EGFR signaling and reprograms cellular response via Mre-11
Authors: Y Volman, R Hefetz, E Galun, J Rachmilewi
Scientific Reports, 2022-04-06;12(1):5760.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
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What is the light chain for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog #s MAB9577 and FAB9577, Clone # Hu1?
Catalog #s MAB9577 and FAB9577 have a kappa light chain.
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PBMC derived, CD3-CD28 expanded, T cells were transduced with a CAR-T LV vector encoding also for the EGFR marker. Staining with anti EGFR AF488 (FAB9577G) was used to assess transduction efficiency (see plot).
