Human EGFR (Research Grade Cetuximab Biosimilar) Antibody

Catalog #: MAB9577 Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB9577-100
MAB9577-SP

Best Seller

Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry.

1 Image

All EGFR Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EGFR (Research Grade Cetuximab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects human EGFR based on Cetuximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Cetuximab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu1

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human EGF R/ErbB1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Research Grade Cetuximab Biosimilar) Monoclonal Antibody (Catalog # MAB9577, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

Cetuximab is a biosimilar directed against the extracellular domain of EGFR. This product constitutes the immunogen used for the anti-Idiotype catalog number MAB9626. The biotinylated antibody (catalog number FAB9577B) can be used in flow cytometry or various assay format to measure EGFR expression and can be detected by conjugated streptavidin.

Long Name

Epidermal Growth Factor Receptor

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Alternate Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

What is a Biosimilar antibody?
- A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody. It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody. For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.
Does this Biosimilar antibody have the same sequence as its corresponding therapeutic antibody?
- This Biosimilar antibody has the same variable region sequence as the therapeutic antibody, but the constant region is not the same. It contains a proprietary human IgG constant region.
Is the light chain of Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog # MAB9577, kappa or lamba?
- MAB9577 is a kappa light chain IgG1.
What is the light chain for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog #s MAB9577 and FAB9577, Clone # Hu1?
- Catalog #s MAB9577 and FAB9577 have a kappa light chain.