Human eNOS Antibody

Catalog #: AF950
11 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF950
AF950-SP

Detection of Human eNOS by Western Blot.

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All eNOS Antibodies

Product Details

Citations (11)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human eNOS Antibody Summary

Species Reactivity

Human

Specificity

Detects human eNOS in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human eNOS

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human placenta subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

0.3 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human eNOS by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothlial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eNOS Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eNOS at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunoprecipitation

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Immunoprecipitation of Human eNOS. eNOS was immunoprecipitated from lysates (1 x 10⁶cells) of HUVEC human umbilical vein endothelial cells following incubation with 0.3 µg Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). Immunoprecipitated eNOS was detected by Western blot using 1 µg/mL Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). View our recommended buffer recipes for immunoprecipitation.

Simple Western

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Detection of Human eNOS by Simple Western^TM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for eNOS at approximately 139 kDa (as indicated) using 10 µg/mL of Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: eNOS

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme encoded by the NOS3 gene. Endothelial NOS generates nitric oxide in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction and platelet aggregation. A constitutive calcium dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of the Golgi apparatus within cells.

Long Name

Endothelial Nitric Oxide Synthase

Entrez Gene IDs

4846 (Human); 18127 (Mouse)

Alternate Names

Constitutive NOS; EC 1.14.13.39; ECNOS; Endothelial NOS; endothelial; eNOS; eNOScNOS; nitric oxide synthase 3 (endothelial cell); NOS type III; NOS3; NOSIII

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human eNOS Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Elderberry extract improves molecular markers of endothelial dysfunction linked to atherosclerosis
Authors: Festa J, Hussain A, Hackney A et al.
Food Science & Nutrition
Soluble pathogenic tau enters brain vascular endothelial cells and drives cellular senescence and brain microvascular dysfunction in a mouse model of tauopathy
Authors: Hussong, SA;Banh, AQ;Van Skike, CE;Dorigatti, AO;Hernandez, SF;Hart, MJ;Ferran, B;Makhlouf, H;Gaczynska, M;Osmulski, PA;McAllen, SA;Dineley, KT;Ungvari, Z;Perez, VI;Kayed, R;Galvan, V;
Nature communications
Species: Human
Sample Types: Whole Cells
Applications: ICC
Oscillatory Shear Stress Induces Oxidative Stress via TLR4 Activation in Endothelial Cells
Authors: Z Wang, F Wang, X Kong, X Gao, Y Gu, J Zhang
Mediators Inflamm., 2019-06-17;2019(0):7162976.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-Fr, Western Blot
A Novel Triple-Cell Two-Dimensional Model to Study Immune-Vascular Interplay in Atherosclerosis
Authors: Jonathan Noonan, Gianluca Grassia, Neil MacRitchie, Paul Garside, Tomasz J. Guzik, Angela C. Bradshaw et al.
Frontiers in Immunology
High glutathionylation of placental endothelial nitric oxide synthase in preeclampsia
Authors: P Guerby, A Swiader, N Augé, O Parant, C Vayssière, K Uchida, R Salvayre, A Negre-Salv
Redox Biol, 2019-01-26;22(0):101126.
Species: Human
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Immunoprecipitation
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??.
Authors: Bing Liu, Lili Xu, Xinming Yu, Wei Li, Xiaozhi Sun, Shun Xiao, Mingjin Guo, Haofu Wang
Molecular Medicine Reports, 2018-06-20;0(0):1791-3004.
Species: Human
Sample Types: Cell Lysate, Cell Lysates
Applications: Western Blot
Memantine protects against ischemia/reperfusion-induced brain endothelial permeability
Authors: Y Liu, Y Huang, Y Xu, P Qu, M Wang
IUBMB Life, 2018-03-15;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
VEGF Receptor-2–Linked PI3K/Calpain/SIRT1 Activation Mediates Retinal Arteriolar Dilations to VEGF and Shear Stress
Authors: Travis W. Hein, Robert H. Rosa, Yi Ren, Wenjuan Xu, Lih Kuo
Investigative Opthalmology & Visual Science
Shear stress-induced ATP-mediated endothelial constitutive nitric oxide synthase expression in human lymphatic endothelial cells.
Authors: Kawai Y, Yokoyama Y, Kaidoh M, Ohhashi T
Am. J. Physiol., Cell Physiol., 2009-12-30;298(3):C647-55.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Regional differences in prostaglandin E2 and nitric oxide production in the knee meniscus in response to dynamic compression.
Authors: Hennerbichler A, Fermor B, Hennerbichler D, Weinberg JB, Guilak F
Biochem. Biophys. Res. Commun., 2007-05-14;358(4):1047-53.
Species: Porcine
Sample Types: Cell Lysates
Applications: Western Blot

Cigarette smoke induces persisting increases of vasoactive mediators in pulmonary arteries.
Authors: Wright JL, Tai H, Churg A
Am. J. Respir. Cell Mol. Biol., 2004-07-08;31(5):501-9.
Species: Guinea Pig
Sample Types: Whole Tissue
Applications: IHC-P