Human ENPP-1 APC-conjugated Antibody

Catalog #: FAB6136A Datasheet / COA / SDS

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FAB6136A

Detection of ENPP‑1 in HepG2 Human Cell Line by Flow Cytometry.

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Human ENPP-1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ENPP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human ENPP-2 and recombinant mouse ENPP-2 is observed after removal of cross-reactivity with human ENPP-2.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ENPP-2-1-2
Asp49-Trp144 (ENPP-2), Val191-Leu591 (ENPP-1), Asn532-Ile863 (ENPP-2). Cross-reactivity with human ENPP-2 was removed from the final product.
Accession # Q13822 (ENPP-2) P22413 (ENPP-1)

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ENPP‑1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human ENPP-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6136A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ENPP-1

Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP-1) is a transmembrane glycoprotein that hydrolyzes nucleotides and nucleotide derivatives with the formation of nucleotide-5’-monophosphates. It is inserted into the plasma membrane by an N‑terminal transmembrane domain. Human ENPP-1 has a small N-terminal cytoplasmic domain and a large C-terminal region containing two somatomedin B-like domains, a catalytic domain and a nuclease-like domain in the extracellular space (1). Defects in the ENPP-1 gene cause arterial calcification and bone mineralization abnormalities (2). ENPP-1 polymorphism or overexpression is also associated with obesity, type II diabetes and insulin resistance, which makes modulation of ENPP-1 activity one of the targets to treat insulin resistance and related diseases (1).

References

Goldfine, I.D. et al. (2010) Endocrine Reviews. 29:62.
Hessle, L. et al. (2002) Proc. Natl. Acad. Sci. 99:9445.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 1

Entrez Gene IDs

5167 (Human); 18605 (Mouse)

Alternate Names

ectonucleotide pyrophosphatase/phosphodiesterase 1; E-NPP 1; ENPP1; ENPP-1; Ly-41 Antigen; M6S1; M6S1NPP1; Membrane component chromosome 6 surface marker 1; membrane component, chromosome 6, surface marker 1; NPP1; NPPS; NPPSalkaline phosphodiesterase 1; PC1; PC-1; PCA1; PCA1ARHR2; PDNP1; PDNP1ectonucleotide pyrophosphatase/phosphodiesterase family member 1; Phosphodiesterase I/nucleotide pyrophosphatase 1; plasma-cell membrane glycoprotein 1; Plasma-cell membrane glycoprotein PC-1