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Human EOMES APC-conjugated Antibody

Catalog #: IC6166A
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC6166A
Detection of EOMES in Mesendoderm-differentiated BG01V Human Cells by Flow Cytometry.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EOMES APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human EOMES in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Brachyury, rhEOMES (aa 1-115), recombinant mouse EOMES (aa 1-126), rhTBX2, 3, 5, 6, 18, or 20 is observed.
Source
Monoclonal Mouse IgG2B Clone # 644730
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human EOMES
Gly471-Pro686
Accession # O95936
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of EOMES antibody in Mesendoderm-differentiated BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of EOMES in Mesendoderm-differentiated BG01V Human Cells by Flow Cytometry. Mesendoderm-differentiated BG01V human embryonic stem cells were stained with Mouse Anti-Human EOMES APC-conjugated Monoclonal Antibody (Catalog # IC6166A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EOMES

EOMES (Eomesodermin; Eo from Greek meaning “dawn”/early in mesoderm; also TBR2) is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. 

Long Name
Eomesodermin Homolog
Entrez Gene IDs
8320 (Human); 13813 (Mouse); 316052 (Rat)
Alternate Names
EOMES; eomesodermin (Xenopus laevis) homolog; Eomesodermin; t box, brain, 2; T-box brain protein 2; T-box brain2; TBR2; TBR-2; TBR2eomesodermin homolog; T-brain-2

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Citations for Human EOMES APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Targeting AURKA in treatment of peritoneal tumor dissemination in gastrointestinal cancer
    Authors: H Ozawa, H Imazeki, Y Ogiwara, H Kawakubo, K Fukuda, Y Kitagawa, C Kudo-Saito
    Translational Oncology, 2021-12-10;16(0):101307.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Hobit expression by a subset of human liver-resident CD56(bright) Natural Killer cells
    Authors: S Lunemann, G Martrus, H Goebels, T Kautz, A Langenecke, W Salzberger, M Koch, M J Bunders, B Nashan, KPJM van Gisber, M Altfeld
    Sci Rep, 2017-07-27;7(1):6676.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Mesendogen, a novel inhibitor of TRPM6, promotes mesoderm and definitive endoderm differentiation of human embryonic stem cells through alteration of magnesium homeostasis
    Authors: Yijie Geng, Bradley Feng
    Heliyon

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