Human EPCR Antibody

Catalog #: MAB22451
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB22451
MAB22451-SP

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Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EPCR Antibody Summary

Species Reactivity

Human

Specificity

Detects human EPCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse EPCR is observed.

Source

Monoclonal Mouse IgG_2B Clone # 304519

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EPCR
Ser18-Ser210
Accession # Q9UNN8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human EPCR

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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EPCR in Human Skin. EPCR was detected in immersion fixed paraffin-embedded sections of human skin using 25 µg/mL Mouse Anti-Human EPCR Monoclonal Antibody (Catalog # MAB22451) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EPCR

Protein C is a vitamin K-dependent serine protease that plays a major role in blood coagulation. Binding of Protein C to EPCR (endothelial protein C receptor) leads to the proteolytic activation of PAR1 (protease-activated receptor 1) on endothelial cells and subsequent up-regulation of Protein C-induced genes. EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. The extracellular domain of human and mouse EPCR shares approximately 61% amino acid sequence homology.

Long Name

Endothelial Protein C Receptor

Entrez Gene IDs

10544 (Human); 19124 (Mouse)

Alternate Names

APC receptor; CCCA; CCD41; CCD41centrosome-associated protein; CD201 antigen; CD201; centrocyclin; Endothelial cell protein C receptor; endothelial protein C receptor; EPCR; EPCRMGC23024; PROCR; protein C receptor, endothelial

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human EPCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Identification of two major autoantigens negatively regulating endothelial activation in Takayasu arteritis
Authors: T Mutoh, T Shirai, T Ishii, Y Shirota, F Fujishima, F Takahashi, Y Kakuta, Y Kanazawa, A Masamune, Y Saiki, H Harigae, H Fujii
Nat Commun, 2020-03-09;11(1):1253.
Species: Human
Sample Types: Cell Culture Lysates
Applications: Western Blot
Endothelial protein C receptor is overexpressed in colorectal cancer as a result of amplification and hypomethylation of chromosome 20q
Authors: N Lal, CR Willcox, A Beggs, P Taniere, A Shikotra, P Bradding, R Adams, D Fisher, G Middleton, C Tselepis, BE Willcox
J Pathol Clin Res, 2017-07-14;3(3):155-170.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P