Human EPCR Antibody

Catalog #: AF2245
10 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2245
AF2245-SP

Detection of Human EPCR by Western Blot.

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All EPCR Antibodies

Product Details

Citations (10)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EPCR Antibody Summary

Species Reactivity

Human

Specificity

Detects human EPCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse EPCR is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EPCR
Ser18-Ser210
Accession # Q9UNN8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

HUVEC human umbilical vein endothelial cells

Immunohistochemistry

1-15 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human EPCR by Western Blot. Western blot shows lysates of human skin tissue, human placenta tissue, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EPCR at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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EPCR in Human Liver. EPCR was detected in immersion fixed paraffin-embedded sections of human liver using 1.7 µg/mL Goat Anti-Human EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2245) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EPCR

EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. Protein C is a vitamin K-dependent serine protease that plays a major role in blood coagulation. Binding of Protein C to EPCR leads to the proteolytic activation of PAR1 (protease-activated receptor 1) on endothelial cells and subsequent up-regulation of Protein C-induced genes. The extracellular domain of human and mouse EPCR shares approximately 61% amino acid sequence homology.

Long Name

Endothelial Protein C Receptor

Entrez Gene IDs

10544 (Human); 19124 (Mouse)

Alternate Names

APC receptor; CCCA; CCD41; CCD41centrosome-associated protein; CD201 antigen; CD201; centrocyclin; Endothelial cell protein C receptor; endothelial protein C receptor; EPCR; EPCRMGC23024; PROCR; protein C receptor, endothelial

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human EPCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients
Authors: L Di Pietro, F Giacalone, E Ragozzino, V Saccone, F Tiberio, M De Bardi, M Picozza, G Borsellino, W Lattanzi, E Guadagni, S Bortolani, G Tasca, E Ricci, O Parolini
Cell Death & Disease, 2022-09-16;13(9):793.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
EPCR-PAR1 biased signaling regulates perfusion recovery and neovascularization in peripheral ischemia
Authors: ML Bochenek, R Gogiraju, S Gro beta mann, J Krug, J Orth, S Reyda, GS Georgiadis, H Spronk, S Konstantin, T Münzel, JH Griffin, PS Wild, C Espinola-K, W Ruf, K Schäfer
JCI Insight, 2022-07-22;0(0):.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot
Interplay of Plasmodium falciparum and thrombin in brain endothelial barrier disruption
Authors: M Avril, M Benjamin, MM Dols, JD Smith
Sci Rep, 2019-09-11;9(1):13142.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes
Authors: G Ecklu-Mens, RW Olsen, A Bengtsson, MF Ofori, L Hviid, ATR Jensen, Y Adams
PLoS ONE, 2018-10-09;13(10):e0204177.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC
Infected erythrocytes expressing DC13 PfEMP1 differ from recombinant proteins in EPCR-binding function
Authors: Y Azasi, G Lindergard, A Ghumra, J Mu, LH Miller, JA Rowe
Proc. Natl. Acad. Sci. U.S.A., 2018-01-16;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay
Interaction between Endothelial Protein C Receptor and Intercellular Adhesion Molecule 1 to Mediate Binding of Plasmodium falciparum-Infected Erythrocytes to Endothelial Cells
MBio, 2016-07-12;7(4):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Cellular regulation of blood coagulation: a model for venous stasis.
Authors: Campbell JE, Brummel-Ziedins KE, Butenas S
Blood, 2010-09-23;116(26):6082-91.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
Authors: Gunawardana CG, Kuk C, Smith CR
J. Proteome Res., 2009-10-01;8(10):4705-13.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Aldosterone modifies hemostasis via upregulation of the protein-C receptor in human vascular endothelium.
Authors: Ducros E, Berthaut A, Mirshahi SS, Faussat AM, Soria J, Agarwal MK, Mirshahi M
Biochem. Biophys. Res. Commun., 2008-06-13;373(2):192-6.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Dot Blot, Flow Cytometry, ICC
Protein C is an autocrine growth factor for human skin keratinocytes.
Authors: Xue M, Campbell D, Jackson CJ
J. Biol. Chem., 2007-02-09;282(18):13610-6.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P