Human Ephrin-A5 Antibody Summary
Gln21-Asn203
Accession # P52803
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Ephrin‑A5 in Human Melanoma. Ephrin-A5 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human Ephrin-A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3743) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ephrin-A5
Ephrin-A5, also known as AL-1, RAGS, LERK-7, and EFL-5 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A5 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphA8 (2, 3). The extracellular domains of murine and human Ephrin-A5 share 99% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).
- Eph Nomenclature Committee [letter] (1997) Cell 90:403.
- Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
- Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Product Datasheets
Citations for Human Ephrin-A5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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ADAM10-cleaved ephrin-A5 contributes to prostate cancer metastasis
Authors: C Cai, M Zhang, L Liu, H Zhang, Y Guo, T Lan, Y Xu, P Ma, S Li
Cell Death & Disease, 2022-05-12;13(5):453.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation -
EphA4 may contribute to microvessel remodeling in the hippocampal CA1 and CA3 areas in a mouse model of temporal lobe epilepsy
Mol Med Rep, 2016-12-09;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Role of synaptic plasticity and EphA5-ephrinA5 interaction within the ventromedial hypothalamus in response to recurrent hypoglycemia.
Authors: Szepietowska, Barbara, Horvath, Tamas L, Sherwin, Robert S
Diabetes, 2013-11-12;63(3):1140-7.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
EphA5-EphrinA5 interactions within the ventromedial hypothalamus influence counterregulatory hormone release and local glutamine/glutamate balance during hypoglycemia.
Authors: Szepietowska B, Zhu W, Czyzyk J, Eid T, Sherwin R
Diabetes, 2012-12-28;62(4):1282-8.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
beta3GnT2 null mice exhibit defective accessory olfactory bulb innervation.
Authors: Henion T, Madany P, Faden A, Schwarting G
Mol Cell Neurosci, 2012-09-21;52(0):73-86.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
EphrinA5 acts as a tumor suppressor in glioma by negative regulation of epidermal growth factor receptor.
Authors: Li JJ, Liu DP, Liu GT, Xie D
Oncogene, 2009-03-09;28(15):1759-68.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-P -
Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction.
Authors: Yumoto N, Wakatsuki S, Kurisaki T, Hara Y, Osumi N, Frisen J, Sehara-Fujisawa A
PLoS ONE, 2008-10-02;3(10):e3322.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping.
Authors: Lim YS, McLaughlin T, Sung TC, Santiago A, Lee KF, O'Leary DD
Neuron, 2008-09-11;59(5):746-58.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
BIG-2 mediates olfactory axon convergence to target glomeruli.
Authors: Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y
Neuron, 2008-03-27;57(6):834-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets.
Authors: Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E
Cell, 2007-04-20;129(2):359-70.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans.
Authors: Janes PW, Saha N, Barton WA, Kolev MV, Wimmer-Kleikamp SH, Nievergall E, Blobel CP, Himanen JP, Lackmann M, Nikolov DB
Cell, 2005-10-21;123(2):291-304.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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