Human ErbB2/Her2 F(ab')2 (Research Grade Trastuzumab Biosimilar) Antibody

Catalog #: MAB11043 Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB11043-100
MAB11043-SP

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Human ErbB2/Her2 F(ab')2 (Research Grade Trastuzumab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects human ErbB2/Her2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Trastuzumab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu5F2

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human ErbB2/Her2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-25 µg/mL

Immersion fixed SK‑BR‑3 human breast cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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ErbB2/Her2 in SK‑BR‑3 Human Cell Line. ErbB2/Her2 was detected in immersion fixed SK‑BR‑3 human breast cancer cell line (positive staining) and THP‑1 human acute monocytic leukemia cell line (negative staining) using Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB11043) at 5 µg/mL for 3 hours at room temperature. Cells were stained using Anti-human-Cy3 antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ErbB2/Her2

Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB2). This product constitutes the immunogen used for the anti-Idiotype catalog numbers MAB9547 and MAB95471. The biotinylated antibody (catalog number FAB9589B) can be used in flow cytometry or various assay formats to measure Her2 expression and can be detected by conjugated streptavidin.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Alternate Names

CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)