Human FABP5/E-FABP Biotinylated Antibody

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BAF3077
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Human FABP5/E-FABP Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human FABP5/E-FABP in Western blots. In Western blots, approximately 30% cross-reactivity with recombinant mouse (rm) FABP5 is observed and less than 5% cross-reactivity with recombinant human (rh) FABP1, rhFABP2, rhFABP3, rhFABP4, rhFABP6, rhFABP7, and rmFABP9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FABP5/E-FABP
Ala2-Glu135
Accession # Q01469
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FABP5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP5/E-FABP

Human FABP-5, also known as epidermal fatty acid binding protein (E-FABP), is a 15 kDa member of a cytosolic fatty acid binding protein superfamily. It is associated with keratinocytes and adipocytes and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors. The amino acid sequence of human FABP5 is 80%, 81% and 92% identical to that of mouse, rat and bovine FABP5, respectively.

Long Name
Fatty Acid-Binding Protein 5
Entrez Gene IDs
2171 (Human); 16592 (Mouse); 140868 (Rat)
Alternate Names
EFABP; E-FABP; E-FABPPAFABP; FABP5; fatty acid binding protein 5 (psoriasis-associated); Fatty acid-binding protein 5; PA-FABPepidermal; Psoriasis-associated fatty acid-binding protein homolog

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