Human Fc gamma RIII (CD16) Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB43251G Datasheet / COA / SDS

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FAB43251G-100UG

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Human Fc gamma RIII (CD16) Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fc gamma RIIIA (CD16a) in direct ELISAs. In direct ELISAs, 60% cross-reactivity with Fc gamma RIIIB (CD16b) is observed.

Source

Monoclonal Mouse IgG_2A Clone # 1001005

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human Fc gamma RIIIA/CD16a
Gly17-Gln208
Accession # P08637

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

Human PBMCs

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fc gamma RIII (CD16)

Fc gamma RIIIa is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes (1, 2). In humans, it is a 50-70 kDa type I transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages (1). Fc gamma RIIIb is highly related, sharing 97% amino acid (aa) identity within the extracellular domain (ECD), but is a GPI-linked receptor expressed on human neutrophils and eosinophils (1, 2). The ECD of Fc gamma RIIIa shares 63%, 61%, 65%, 59% and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIa, feline CD16, bovine CD16 and porcine Fc gamma RIIIb paralogs, respectively. The Fc gamma RIIIa cDNA encodes 254 aa including a 16 aa signal sequence, 191 aa ECD with two C2-type Ig-like domains and five potential N-glycosylation sites, a 22 aa transmembrane (TM) sequence and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism creates high binding (176V) and low binding (176F) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (3, 4). Catalog # 4325-FC is expressed as the 176V isoform of Fc gamma RIIIa. Fc gamma RIIIa surface expression requires interaction of an accessory chain, either the common gamma -chain or CD3 zeta (5, 6). Glycosylation patterns, electrophoretic mobility and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIa (7). The ECD of both Fc gamma RIIIa and b can be proteolytically cleaved and retain binding activity in soluble form (8-11). In monocytes and macrophages, activation and phagocytosis can trigger Fc gamma RIIIa release (11). Soluble Fc gamma RIII can be detected in normal plasma and is increased in rheumatoid arthritis and in coronary artery diseases (9, 10).

References

Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19.
Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481.
Wu, J. et al. (1997) J. Clin. Invest. 100:1059.
Dall’Ozzo, S. et al. (2004) Cancer Res. 64:4664.
Kim, M.-K. et al. (2003) Blood 101:4479.
Lanier, L.L. et al. (1989) Nature 342:803.
Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.
Li, P. et al. (2007) J. Biol. Chem. 282:6210.
Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
Masuda, M. et al. (2006) Atherosclerosis 188:377.
Webster, N.L. et al. (2006) J. Leukoc. Biol. 79:294.

Long Name

Fc gamma Receptor III

Entrez Gene IDs

14131 (Mouse); 102140945 (Cynomolgus Monkey)

Alternate Names

CD16; CD16A; Fc fragment of IgG receptor IIIa; Fc gamma RIII; FCG3; FCGR3; FcgRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20

Product Datasheets

Product Datasheet

COA

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.