Human FCRL1/FcRH1 PE-conjugated Antibody Summary
Ala17-His304
Accession # Q96LA6
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of FCRL1/FcRH1 in Human Blood B cells by Flow Cytometry. Human blood B cells were stained with Mouse Anti-Human FCRL1/FcRH1 PE-conjugated Monoclonal Antibody (Catalog # FAB2049P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FCRL1/FcRH1
Fc Receptor-Like 1 (FCRL1), also known as FcRH1, and IRTA5, is an approximately 50 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL1 binds to purified human IgG. Mature human FCRL1 consists of a 291 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 101 aa cytoplasmic domain with two immunotyrosine activation motifs (ITIMs) (3, 4). A charged glutamic acid within the transmembrane segment may mediate association with other signaling proteins. Alternative splicing may generate an isoform that lacks the transmembrane segment and an isoform that largely consists of the first two Ig-like domains (5). Mouse FCRL1 contains only two Ig-like domains, but it shares 62% aa sequence identity with homologous regions of the human FCRL1 ECD. FCRL1 is expressed on pre-B cells and naive B cells (6, 7). It is down‑regulated upon B cell activation but up‑regulated on memory B cells (6, 8). FCRL1 is expressed on many B cell lymphoma and leukemia tumor cells with the exception of B cell acute lymphoblastic leukemia (9‑11). Antibody crosslinking of FCRL1 triggers its tyrosine phosphorylation and augments B cell proliferation induced by the B cell receptor (6).
- Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525.
- Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
- Miller, I. et al. (2002) Blood 99:2662.
- Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
- Accession # Q96LA6.
- Leu, C.-M. et al. (2005) Blood 105:1121.
- Li, F.J. et al. (2008) Blood 112:179.
- Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
- Du, X. et al. (2008) Blood 111:338.
- Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
- Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.
Product Datasheets
Citation for Human FCRL1/FcRH1 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Evaluation of CD307a expression patterns during normal B-cell maturation and in B-cell malignancies by flow cytometry
Authors: M Auat, CC Cardoso, IM Santos-Pir, RCM Rudolf-Oli, C Matiollo, BG Lange, JP da Silva, GC Dametto, MM Pirolli, MDHP Colombo, MC Santos-Sil
Cytometry B Clin Cytom, 2018-03-09;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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