Human FCRLB/FCRY Antibody

Catalog #: AF4705 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4705
AF4705-SP

Detection of Human FCRLB/FCRY by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FCRLB/FCRY Antibody Summary

Species Reactivity

Human

Specificity

Detects human FCRLB/FCRY in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) FCRLB/FCRY is observed, and less than 1% cross-reactivity with recombinant human FCRN and rmFCRN is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FCRLB/FCRY
Ala18-Ser426
Accession # Q6BAA4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FCRLB/FCRY by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human FCRLB/FCRY Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4705) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FCRLB/FCRY at approximately 57-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRLB/FCRY

FCRLB, also known as FCRL2, FCRY, and FREB2, is a 57-60 kDa protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified human IgG with high affinity. Human FCRLB shares 82% and 85% amino acid sequence identity with mouse and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1 - 3). The stalk region also contains di-Leu motifs and an unpaired cysteine (1 - 3). Alternative splicing generates isoforms with deletions in the putative signal peptide and substitutions and/or truncations in the third Ig-like domain (3). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (3, 4). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (3, 4). It is upregulated in B cells by BAFF and LPS (5). Among non-hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 5). It is preferentially expressed in nonproliferating cells and at the onset of apoptosis (4, 5).

References

Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
Chikaev, N.A. et al. (2005) Genomics 85:264.
Wilson, T.J. and M. Colonna (2005) Genes Immun. 6:341.
Masuda, K. et al. (2005) Gene 363:32.

Long Name

Fc Receptor-like B

Entrez Gene IDs

127943 (Human); 435653 (Mouse); 498275 (Rat)

Alternate Names

Fc receptor homolog expressed in B cells protein 2; Fc receptor like 2; Fc receptor-like and mucin-like 2; Fc receptor-like and mucin-like protein 2; Fc receptor-like B; Fc receptor-like protein 2; Fc receptor-related protein Y; FcRL2; FCRL2FCRLM2; FCRLB; FCRLM2; FCRLY; FCRY; FLJ31052; FREB2; FREB-2; FREB-2FcRY; RP11-474I16.6