Human FGF-6 Antibody

Catalog #: AF238
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF238
AF238-SP

Cell Proliferation Induced by FGF‑6 and Neutralization by Human FGF‑6 Antibody.

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All FGF-6 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF-6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGF-6 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human (rh) FGF-4 is observed, less than 5% cross-reactivity with rhFGF-9 is observed, and less than 2% cross-reactivity with FGF-acidic, rhFGF-5, and rhFGF-7 is observed. In direct ELISAs, less than 1% cross-reactivity with rh beta -ECGF, recombinant mouse (rm) FGF-8b, and rmFGF-8c is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FGF-6
Gly41-Ile208
Accession # P10767

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human FGF‑6 (Catalog # 238-F6)

Neutralization

Measured by its ability to neutralize FGF‑6-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND₅₀) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human FGF‑6 and 1 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by FGF‑6 and Neutralization by Human FGF‑6 Antibody. Recombinant Human FGF-6 (Catalog # 238-F6) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF238). The ND₅₀ is typically 0.2-1 µg/mL in the presence of heparin (1 µg/mL).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-6

Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25-28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature human FGF-6 is a 171 aa protein that shares 94% aa sequence identity with mouse and rat FGF-6 (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5‑7). FGF-6 expression is restricted to skeletal muscle during development although it can be upregulated in prostate cancer and Kaposi sarcoma (7-9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).

References

Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
Marics, I. et al. (1989) Oncogene 4:335.
Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
Pizette, S. et al. (1991) Cell Growth Differ. 2:561.
Asada, M. et al. (1999) Growth Factors 16:293.
Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
de Lapeyriere, O. et al. (1993) Development 118:601.
Li, J. et al. (1993) Cancer 72:2253.
Floss, T. et al. (1997) Genes Dev. 11:2040.
Grass, S. et al. (1996) Development 122:141.

Long Name

Fibroblast Growth Factor 6

Entrez Gene IDs

2251 (Human); 14177 (Mouse); 170700 (Rat)

Alternate Names

FGF6; FGF-6; fibroblast growth factor 6; HBGF-6; Heparin secretory-transforming protein 2; Heparin-binding growth factor 6; HST2; HST-2; HSTF2; HSTF-2

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Citations for Human FGF-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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FGF6 mediated expansion of a resident subset of cells with SP phenotype in the C2C12 myogenic line.
Authors: Israeli D, Benchaouir R, Ziaei S, Rameau P, Gruszczynski C, Peltekian E, Danos O, Garcia L
J. Cell. Physiol., 2004-12-01;201(3):409-19.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Embryonic myogenesis pathways in muscle regeneration.
Authors: Zhao P, Hoffman EP
Dev. Dyn., 2004-02-01;229(2):380-92.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC