Human FGF acidic/FGF1 Antibody

Catalog #: MAB232 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB232
MAB232-SP

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Human FGF acidic/FGF1 Antibody Summary

Species Reactivity

Human

Specificity

Detects rhFGF acidic/FGF1 in direct ELISAs and Western blots. Does not cross-react with any other family members tested.¹¹rhFGF3, rhFGF4, rhFGF5, rhFGF6, rhFGF7, rhFGF8b, rmFGF8b, rmFGF8c, rhFGF9, rhFGF10, rmFGF15, rhFGF17, rhFGF18, rhFGF19, bFGF-acidic, bFGF basic, rhFGF basic

Source

Monoclonal Mouse IgG₁ Clone # 3117

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human FGF acidic/FGF1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human FGF acidic aa 16-155 (Catalog # 232-FA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF acidic/FGF1

FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist.

Long Name

Fibroblast Growth Factor acidic

Entrez Gene IDs

2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)

Alternate Names

AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1