Human Fibroblast Activation Protein alpha /FAP Biotinylated Antibody
Human Fibroblast Activation Protein alpha /FAP Biotinylated Antibody Summary
Leu26-Asp760
Accession # Q12884
Applications
Human Fibroblast Activation Protein alpha /FAP Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Fibroblast Activation Protein alpha /FAP by Western Blot. Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein a/FAP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3715) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for Fibroblast Activation Protein a/FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibroblast Activation Protein alpha/FAP
FAP (also known as seprase) is a Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (1). FAP has substrate specificity similar to dipeptidyl peptidase IV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I collagen (2). The enzymatically active form of FAP is a dimer (3). FAP has a restricted tissue distribution. It is not detectable in normal tissues or resting fibroblasts, but is highly expressed on reactive stromal fibroblasts in epithelial cancers (4), in granulation tissue during wound healing, and in bone and soft tissue sarcomas (5). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair.
- Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
- Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
- Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
- Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
- Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
Product Datasheets
Citations for Human Fibroblast Activation Protein alpha /FAP Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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ADAM12 expression is upregulated in cancer cells upon radiation and constitutes a prognostic factor in rectal cancer patients following radiotherapy
Authors: Piotrowski, KB;Blasco, LP;Samsøe-Petersen, J;Eefsen, RL;Illemann, M;Oria, VO;Campos, KIA;Lopresti, AM;Albrechtsen, R;Sørensen, CS;Sun, XF;Kveiborg, M;Gnosa, S;
Cancer gene therapy
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chitooligosaccharides Improve the Efficacy of Checkpoint Inhibitors in a Mouse Model of Lung Cancer
Authors: AZ Johansen, M Carretta, ML Thorseth, S Khan, KY Fjæstad, CB Brøchner, H Linder, C Ankjærgaar, M Donia, I Chen, DL Nielsen, CP Behrens, DH Madsen
Oncogene, 2022-05-12;14(5):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Fibroblast activation protein augments progression and metastasis of pancreatic ductal adenocarcinoma
Authors: A Lo, CP Li, EL Buza, R Blomberg, P Govindaraj, D Avery, J Monslow, M Hsiao, E Puré
JCI Insight, 2017-10-05;2(19):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cancer associated fibroblasts in pancreatic cancer are reprogrammed by tumor-induced alterations in genomic DNA methylation
Cancer Res, 2016-08-05;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Selection
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Since the mouse FAP shares ~90% of the human protein sequence, in our hands this antibody works good also mouse cells.
We are using it for flow cytometry on primary mouse cells in combination with APC Streptavidin (Biolegend, 405207). The staining works properly with a concentration of 1:20 of FAP Biotinylated Antibody and 1:100 of APC Streptavidin.
