Human Fibroblast Activation Protein alpha /FAP PE-conjugated Antibody
Human Fibroblast Activation Protein alpha /FAP PE-conjugated Antibody Summary
Leu26-Asp760
Accession # Q12884
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Fibroblast Activation Protein alpha /FAP in WI‑38 Human Cell Line by Flow Cytometry. WI-38 human lung fibroblast cell line was stained with Mouse Anti-Human Fibroblast Activation Protein a/FAP PE-conjugated Monoclonal Antibody (Catalog # FAB3715P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of Fibroblast Activation Protein alpha /FAP in U87MG cells by Flow Cytometry. U87MG cells were stained with Mouse Anti-Human Fibroblast Activation Protein alpha /FAP PE‑conjugated Monoclonal Antibody (Catalog # FAB3715P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fibroblast Activation Protein alpha/FAP
FAP (also known as Seprase) is a 97 kDa Type II transmembrane serine protease that is structurally related to Dipeptidyl Peptidase IV (DPPIV) (1). FAP has substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I Collagen (2). The enzymatically active form of FAP is a dimer that migrates at ~170 kDa. It is associated with multiple integral membrane proteins such as Integrin alpha 3 beta 1, UPA and DPPIV (3,4). FAP has a restricted tissue distribution. It is occasionally detected in fibroblasts and pancreatic islet cells, but is highly expressed on reactive stromal fibroblasts in epithelial cancers, in granulation tissue during wound healing, and in bone and soft tissue sarcomas (4-6). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair. The 760 amino acid (aa) human FAP contains a 735 aa extracellular domain that is glycosylated and necessary for activity (4). It shares 90% aa identity with mouse and rat FAP. A reported 672 aa splicing variant diverges prior to the active site charge relay residues at the C-terminus.
- Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
- Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
- Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
- O'Brien, P. and B.F. O'Connor (2008) Biochim. Biophys. Acta 1784:1130.
- Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
- Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
Product Datasheets
Citations for Human Fibroblast Activation Protein alpha /FAP PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Patient-Derived Primary Cancer-Associated Fibroblasts Mediate Resistance to Anti-Angiogenic Drug in Ovarian Cancers
Authors: R Sulaiman, P De, JC Aske, X Lin, A Dale, N Koirala, K Gaster, LR Espaillat, D Starks, N Dey
Biomedicines, 2023-01-01;11(1):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Prostacyclin Released by Cancer-Associated Fibroblasts Promotes Immunosuppressive and Pro-Metastatic Macrophage Polarization in the Ovarian Cancer Microenvironment
Authors: L Sommerfeld, I Knuth, F Finkernage, J Pesek, WA Nockher, JM Jansen, U Wagner, A Nist, T Stiewe, S Müller-Brü, R Müller, S Reinartz
Cancers, 2022-12-13;14(24):.
Species: Human
Sample Types: Whole Cell
Applications: Flow Cytometry -
Differential Marker Expression between Keratinocyte Stem Cells and Their Progeny Generated from a Single Colony
Authors: D Ali, D Alhattab, H Jafar, M Alzubide, N Sharar, S Bdour, A Awidi
International Journal of Molecular Sciences, 2021-10-06;22(19):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
An ex vivo tumor fragment platform to dissect response to PD-1 blockade in cancer
Authors: P Voabil, M de Bruijn, LM Roelofsen, SH Hendriks, S Brokamp, M van den Br, A Broeks, J Sanders, P Herzig, A Zippelius, CU Blank, KJ Hartemink, K Monkhorst, JBAG Haanen, TN Schumacher, DS Thommen
Nature Medicine, 2021-07-08;27(7):1250-1261.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Destabilization of NOXA mRNA as a common resistance mechanism to targeted therapies
Authors: J Montero, C Gstalder, DJ Kim, D Sadowicz, W Miles, M Manos, JR Cidado, J Paul Secri, AE Tron, K Flaherty, F Stephen Ho, CH Yoon, A Letai, DE Fisher, R Haq
Nat Commun, 2019-11-14;10(1):5157.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Isolation of Fibroblast-Activation Protein-Specific Cancer-Associated Fibroblasts
Authors: Y Huang, S Zhou, Y Huang, D Zheng, Q Mao, J He, Y Wang, D Xue, X Lu, N Yang, Y Zhao
Biomed Res Int, 2017-08-14;2017(0):4825108.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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