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Human Flt-3 Ligand/FLT3L Biotinylated Antibody

Catalog #: BAF308
4 Citations Datasheet / COA / SDS
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BAF308
Product Details
Citations (4)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Flt-3 Ligand/FLT3L Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Flt-3 Ligand/FLT3L in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) PlGF, recombinant mouse Flt-3 Ligand/FLT3L, rhVEGF and rhFlt-1 Fc chimera is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Flt-3 Ligand/FLT3L
Thr27-Pro185
Accession # AAA17999
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Flt-3 Ligand/FLT3L (Catalog # 308-FK)

Human Flt-3 Ligand/FLT3L Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Flt-3 Ligand/FLT3L Antibody (Catalog # MAB608)

Standard: Recombinant Human Flt-3 Ligand/FLT3L Protein (Catalog # 308-FKN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1 - 3). Mature human Flt-3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail (4 - 7). Within the ECD, human Flt-3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively. Human and mouse Flt-3 Ligand show cross-species activity (4 - 6). Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain (4, 8). Alternate splicing of human Flt-3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain (4, 5, 8). Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3, 4, 6, 7). Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 9). It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4 - 6). It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation (1, 10). Animal studies also show Flt‑3 Ligand to reduce the severity of experimentally induced allergic inflammation (11).

References
  1. Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
  2. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
  3. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
  4. Hannum, C. et al. (1994) Nature 368:643.
  5. Lyman, S.D. et al. (1994) Blood 83:2795.
  6. Lyman, S.D. et al. (1993) Cell 75:1157.
  7. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
  8. McClanahan, T. et al. (1996) Blood 88:3371.
  9. Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
  10. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
  11. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.
Long Name
fms-like Tyrosine Kinase 3 Ligand
Entrez Gene IDs
2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)
Alternate Names
FL; FLG3L; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine

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Citations for Human Flt-3 Ligand/FLT3L Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Efficacy and safety of CDX-301, recombinant human Flt3L, at expanding dendritic cells and hematopoietic stem cells in healthy human volunteers.
    Authors: Anandasabapathy N, Breton G, Hurley A, Caskey M, Trumpfheller C, Sarma P, Pring J, Pack M, Buckley N, Matei I, Lyden D, Green J, Hawthorne T, Marsh H, Yellin M, Davis T, Keler T, Schlesinger S
    Bone Marrow Transplant, 2015-04-27;50(7):924-30.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  2. Expression of CCL20 and granulocyte-macrophage colony-stimulating factor, but not Flt3-L, from modified vaccinia virus ankara enhances antiviral cellular and humoral immune responses.
    Authors: Chavan R, Marfatia KA, An IC, Garber DA, Feinberg MB
    J. Virol., 2006-08-01;80(15):7676-87.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. Long-term maintenance of gp120-specific immune responses by genetic vaccination with the HIV-1 envelope genes linked to the gene encoding Flt-3 ligand.
    Authors: Sailaja G, Husain S, Nayak BP, Jabbar AM
    J. Immunol., 2003-03-01;170(5):2496-507.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. Attenuation of vaccinia virus by the expression of human Flt3 ligand
    Authors: Kamila Zurkova, Petr Hainz, Jitka Krystofova, Luda Kutinova, Miloslav Sanda, Sarka Nemeckova
    Virology Journal

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