Human Galectin-4 Antibody Summary
-3, -7, -8, recombinant mouse Galectin-1, -3, -4, or -7 is observed.
Ala2-Ile323
Accession # P56470
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Galectin‑4 by Western Blot. Western blot shows lysates of human colon tissue, COLO 205 human colorectal adenocarcinoma cell line, T84 human colon carcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Galectin-4 Monoclonal Antibody (Catalog # MAB1227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin-4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-4
Galectins are a family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins share structural similarities in their carbohydrate recognition domains (CRD), forming three groups often termed prototype (one CRD), tandem-repeat (two CRDs) and chimeric (one CRD, unique N-terminus) (1, 2). All lack classical signal peptides, but are present and active both within and outside of the cell. Galectins are involved in cell adhesion, migration, survival and apoptosis, and are often up- or down-regulated in cancer (1‑3). Galectin-4 is a 36 kDa tandem-repeat galectin found throughout the gastrointestinal tract, but also present in well-differentiated breast and liver carcinomas (3, 4). Each CRD binds a different set of carbohydrate groups, including those found on red cell blood group antigens (3, 5). CRD1 also binds cholesterol 3-sulfate and other sulfatides, which are concentrated within lipid raft membrane microdomains (6, 7). Endocytosed Galectin-4 is thought to play a role in forming the rafts, delivering them to the intestinal apical membrane, and stabilizing highly detergent-resistant "superrafts" (7, 9). Human Galectin-4 shares 76%, 77%, 78% and 80% aa identity with mouse, rat, bovine and porcine Galectin-4, respectively, with the highest identity occurring within the CRDs. A potential splice variant begins at aa 132 and lacks most of the first CRD (10). Galectin-4 expression is concentrated within microvilli in the gastrointestinal epithelium, where it can interact with CD3 and bind activated T cells in the lamina propria during intestinal inflammation (11, 12). Either pro- or anti-inflammatory activity has been shown, depending on the mouse model used. Galectin-4 can also bind lung, spleen and kidney macrophages, although its expression is normally low in these tissues (5).
- Yang, R-Y. et al. (2008) Expert Rev. Mol. Med. 10:e17.
- Elola, M. T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
- Huflejt, M. E. & H. Leffler (2004) Glycoconj. J. 20:247.
- Recreche, H. et al. (1997) Eur. J. Biochem. 248:225.
- Markova, V. et al. (2006) Int. J. Mol. Med. 18:65.
- Ideo, H. et al. (2007) J. Biol. Chem. 282:21081.
- Delacour, D. et al. (2005) J. Cell Biol. 169:491.
- Braccia, A. et al. (2003) J. Biol. Chem. 278:15679.
- Stechly, L. et al. (2009) Traffic Jan 24 e-pub ahead of print.
- Entrez Accession # EAW56820.
- Hokama, A. et al. (2004) Immunity 20:681.
- Paclik, D. et al. (2008) PloS ONE 3:e2629.
Product Datasheets
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AS seen, the antibody is very specific in detecting only galectin-4 and no other non-specific bands.
Specificity: Specific
Sensitivity: Sensitive
Buffer: Milk, BSA, Milk
Dilution: 1:1000
As seen, the antibody is specific and sensitive in detecting 36kDa of galectin-4, while 50 kDa is the antibody bands.
Specificity: Specific
Sensitivity: Sensitive
Buffer: IP buffer
Dilution: 1:1000
The antibody specifically detects the crypt epithelial cells at lower dilutions also. Mouse antibody specific IHC kit was used for staining.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:500
Serves very well to detect the protein using IFC.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:1000
The antibody is very sensitive to detect internal galectin-4 using this antibody.
Specificity: Specific
Sensitivity: Sensitive
Buffer: Fixing buffer
Dilution: 1:100
2D gel was probed for galectin-4 and works very well.
Specificity: Reasonably specific
Sensitivity: Sensitive
Buffer: BSA
Dilution: 1:1000
