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Human Galectin-8 Antibody

Catalog #: MAB1305
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1305
MAB1305-SP
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Galectin-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Galectin-8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Galectin-1, -2, or -7 is observed.
Source
Monoclonal Mouse IgG2A Clone # 210608
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Galectin-8
Met1-Trp317
Accession # O00214
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Galectin‑8 (Catalog # 1305-GA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-8

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain, and the tandem-repeat Galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4).

Galectin-8, also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat-type galectin. Prototype (single CRD) isoforms arising through alternate gene splicing have also been identified (5). Galectin-8 is highly expressed in lung carcinomas, certain forms of prostate carcinomas, as well as other tumor cells. It binds to a subset of cell surface integrins to modulate ECM-integrin interactions. As a soluble ligand, Galectin-8 can inhibit cell adhesion (6). Immobilized Galectin-8, however, has also been shown to promote cell adhesion (7). Human and mouse Galectin-8 share approximately 80% amino acid sequence identity (4).

References
  1. Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2002) Biochimie 83:667.
  4. R&D Systems’ Cytokine Bulletin, Summer, 2002.
  5. Bidon, N. et al. (2001) Gene 274:253.
  6. Hadari, Y. et al. (1995) J. Biol. Chem. 270:3447.
  7. Levy, Y. et al. (2001) J. Biol. Chem. 276:31285.
Entrez Gene IDs
3964 (Human); 56048 (Mouse)
Alternate Names
GAL8; Gal-8; galectin 8; Galectin8; Galectin-8; galectin-8g; lectin, galactoside-binding, soluble, 8; LGALS8; PCTA1; PCTA-1Po66 carbohydrate-binding protein; Po66 carbohydrate binding protein; Po66-CBP; Prostate carcinoma tumor antigen 1

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Citations for Human Galectin-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. SopF, a phosphoinositide binding effector, promotes the stability of the nascent Salmonella-containing vacuole
    Authors: N Lau, AL Haeberle, BJ O'Keeffe, EA Latomanski, J Celli, HJ Newton, LA Knodler
    PLoS Pathog., 2019-07-24;15(7):e1007959.
    Species: Human
    Sample Types: Whole Cells
    Applications: Fluorescence Microscopy
  2. Listeria phospholipases subvert host autophagic defenses by stalling pre-autophagosomal structures.
    Authors: Tattoli, Ivan, Sorbara, Matthew, Yang, Chloe, Tooze, Sharon A, Philpott, Dana J, Girardin, Stephen
    EMBO J, 2013-10-25;32(23):3066-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Survivin enhances motility of melanoma cells by supporting Akt activation and {alpha}5 integrin upregulation.
    Authors: McKenzie JA, Liu T, Goodson AG, Grossman D
    Cancer Res., 2010-08-31;70(20):7927-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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