Human GATA-3 Antibody

Catalog #: MAB26051
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB26051
MAB26051-SP

Detection of Human GATA‑3 by Western Blot.

2 Images

All GATA-3 Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GATA-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GATA-3 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with rhGATA-1, -2, -4, -5, or -6.

Source

Monoclonal Mouse IgG₁ Clone # 291106

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human GATA‑3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GATA-3 Monoclonal Antibody (Catalog # MAB26051) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GATA-3 full length (FL) at approximately 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry

View Larger

GATA‑3 in MCF-7 Human Cell Line. GATA‑3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human GATA‑3 Monoclonal Antibody (Catalog # MAB26051) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-3

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development, and the maintenance of the differentiated state in epithelial cells.

Entrez Gene IDs

2625 (Human); 14462 (Mouse)

Alternate Names

131320; GATA binding protein 3; GATA3; GATA-3; GATA-binding factor 3; GATA-binding protein 3; HDRMGC5199; MGC2346; MGC5445; trans-acting T-cell-specific transcription factor GATA-3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human GATA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

Liver group 2 innate lymphoid cells regulate blood glucose levels through IL-13 signaling and suppression of gluconeogenesis
Authors: M Fujimoto, M Yokoyama, M Kiuchi, H Hosokawa, A Nakayama, N Hashimoto, I Sakuma, H Nagano, K Yamagata, F Kudo, I Manabe, E Lee, R Hatano, A Onodera, K Hirahara, K Yokote, T Miki, T Nakayama, T Tanaka
Nature Communications, 2022-09-15;13(1):5408.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation
Cyclic regulation of T-Bet and GATA-3 in human endometrium.
Authors: Inman D, Kawana K, Schust D
Reprod Sci, 2008-01-01;15(1):83-90.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P