Human GATA-4 Antibody

Name: Human GATA-4 Antibody
Brand: R&D Systems
SKU: MAB2606
Rating: 5 (2 reviews)

Catalog #: MAB2606
2 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2606
MAB2606-SP

Detection of Human GATA‑4 by Western Blot.

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All GATA-4 Antibodies

Product Details

Citations (2)

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Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GATA-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GATA-4 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 532020

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GATA-4
Met27-Phe211
Accession # P43694

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GATA‑4 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human GATA-4 Monoclonal Antibody (Catalog # MAB2606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GATA-4 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-4

GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. GATA-4 contains 2 zinc finger-binding domains and migrates at 56 kDa in SDS-PAGE gels. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.

Entrez Gene IDs

2626 (Human)

Alternate Names

GATA binding protein 4; GATA4; GATA-4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; transcription factor GATA-4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human GATA-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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GATA4 induces liver fibrosis regression by deactivating hepatic stellate cells
Authors: N Arroyo, L Villamayor, I Díaz, R Carmona, M Ramos-Rodr, R Muñoz-Chap, L Pasquali, MG Toscano, F Martin, DA Cano, A Rojas
JCI Insight, 2021-12-08;0(0):.
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot
Electromechanical Conditioning of Adult Progenitor Cells Improves Recovery of Cardiac Function After Myocardial Infarction
Authors: A Llucià-Val, C Soler-Boti, C Gálvez-Mon, S Roura, C Prat-Vidal, I Perea-Gil, B Sanchez, R Bragos, G Vunjak-Nov, A Bayes-Geni
Stem Cells Transl Med, 2016-09-29;6(3):970-981.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr