Human GLP-1 Antibody

Catalog #: MAB10473 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB10473-100
MAB10473-SP

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Human GLP-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GLP-1 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2622B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human GLP-1 synthetic peptide
Accession # P01275

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

0.3-25 µg/mL

Immersion fixed paraffin-embedded sections of human pancreas tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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GLP-1 in Human Pancreas Tissue. GLP-1 was detected in immersion fixed paraffin-embedded sections of human pancreas tissue using Rabbit Anti-Human GLP-1 Monoclonal Antibody (Catalog # MAB10473) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in islets. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLP-1

Glucagon is a preprotein which is cleaved into four distinct peptides, including the hormone GLP-1 (aa 98-128). GLP-1 is a secreted hormone with multiple effects upon the intestine (gastric motility), pancreas (glucose dependent insulin release) and hypothalamic pituitary axis (modulates LH, THS, CRH, ocytocin and vasopressin secretion). It also affects plasma glucagon levels. GLP-1 is expressed in enteroendocrine L cells and neurons of the caudal brainstem which project to the forbain, the amygdala and the hypothalamus. Recent studies show pancreatic intra-islet GLP-1 expression, which is regulated by cytokines, hyperglycemia and cell injury.

Long Name

Glucagon-like Peptide 1

Entrez Gene IDs

2641 (Human)

Alternate Names

glicentin-related polypeptide; GLP1; GLP-1; glucagon-like peptide 1; GRPP