Human Glutaredoxin 3/GLRX3 Antibody

Catalog #: MAB7560 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7560
MAB7560-SP

Detection of Human Glutaredoxin 3/ GLRX3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glutaredoxin 3/GLRX3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glutaredoxin 3/GLRX3 in ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 791431

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Glutaredoxin 3/GLRX3
Asn126-Lys294
Accession # O76003

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Glutaredoxin 3/ GLRX3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Glutaredoxin 3/GLRX3 Monoclonal Antibody (Catalog # MAB7560) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutaredoxin 3/GLRX3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Glutaredoxin 3/GLRX3 in NIH-3T3 Mouse Cell Line. Glutaredoxin 3/GLRX3 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human Glutaredoxin 3/ GLRX3 Monoclonal Antibody (Catalog # MAB7560) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutaredoxin 3/GLRX3

GLRX3 (Glutaredoxin 3; also PICOT, PKC-theta-interacting protein, and Thioredoxin-like protein 2/TXNL2) is a 37-40 kDa member of the multidomain subgroup, monothiol glutaredoxin group of glutaredoxin/GTX proteins. It has widespread expression but is not found ubiquitously. Cells known to express cytosolic GLRX3 are principally epithelial in type, and include breast and adrenal epithelium, pancreatic exocrine gland epithelium, and proximal tubule renal epithelium. Cardiac muscle may also express GLRX3. By inference, it is suggested to be involved in cell proliferation and has also been associated with both PKC-theta regulation and MLP/muscle LIM protein-mediated cardiac contractility. Human GLRX3 is 335 amino acids (aa) in length. It contains an N-terminal thioredoxin-like domain (aa 2-117) that is followed by two PICOT homology domains (aa 144-236 and 237-335). The thioredoxin domain does not possess a typical disulfide, and it is suggested that this domain does not demonstrate redox activity. The PICOT domains will interact with intracellular proteins. GLRX3 exists as both a monomer and homodimer, with the homodimer incorporating two Fe/S clusters into their PICOT domains. These clusters serve as redox sensors within the cell. Over aa 126-294, human GLRX3 shares 98% aa sequence identity with mouse GLRX3.

Entrez Gene IDs

10539 (Human); 30926 (Mouse); 58815 (Rat)

Alternate Names

FLJ11864; GLRX3; GLRX4; Glutaredoxin 3; glutaredoxin 4; glutaredoxin 5; GRX3; GRX4; PICOT; PICOTbA500G10.4; PKC-interacting cousin of thioredoxin; PKCq-interacting protein; thioredoxin-like 2; TXNL2; TXNL3