Human Glypican 2 Antibody

Catalog #: AF2304
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2304
AF2304-SP

All Glypican 2 Antibodies

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Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glypican 2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glypican 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant mouse Glypican 2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Glypican 2
Pro18-Ser553
Accession # Q8N158

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Glypican 2 (Catalog # 2304-GP)

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of mouse embryo (E13.5)

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 25 ng/mL of Recombinant Human Midkine (Catalog # 258-MD) to immobilized Recombinant Human Glypican 2 (Catalog # 2304-GP) coated at 4 µg/mL (100 µL/well). At 200 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 2

The glypicans (GPC) constitute a family of heparan sulfate proteoglycans that are attached to the cell surface by a lycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1‑GPC6). All glypican core proteins contain an N‑terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Based on the degree of their amino acid sequence similarity, two subfamilies of glypicans have been defined. One subfamily (sharing from 35‑63% sequence homology) includes GPC1, 2, 4, and 6, while the second subfamily (sharing 54% sequence identity) includes GPC3 and 5. Proteins between the two subfamilies also share 17‑25% sequence similarity (1‑4). Glypicans are widely expressed in adult and fetal tissues. During embryonic development, the expression level of the various glypicans changes in a stage and tissue specific manner. GPC2, also known as cerebroglycan, is primarily expressed in developing neuronal tissues including the brain, spinal cord, dorsal root ganglia, and cranial nerves. It is found on the tracts of actively growing axons (5). Cell surface GPC2 binds midkine, indicating midkine‑GPC2 interaction may participate in neuronal cell migration and neurite outgrowth (6).

References

Filmus, J and S.B. Selleck (2001) J. Clin. Invest. 108:497.
De Cat, B and G. David (2001) Semin. Cell Dev. Biol. 12:117.
Song, H. and J. Filmus (2002) Biochem. Biophys. Acta 1573:241.
Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
Ivins, J.K. et al. (1997) Dev. Biol. 184:320.
Kurosawa, N. et al. (2001) Glycoconj. J. 18:499.

Entrez Gene IDs

221914 (Human); 71951 (Mouse)

Alternate Names

2410016G05Rik; Cerebroglycan; Glypican 2; GPC2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Glypican 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Doublecortin and Glypican-2 concentrations in the cerebrospinal fluid from infants are developmentally downregulated
Authors: C Brégère, U Fisch, FS Halbeisen, C Schneider, T Dittmar, S Stricker, S Aghlmandi, R Guzman
PLoS ONE, 2023-02-17;18(2):e0279343.
Species: Human
Sample Types: CSF
Applications: ELISA Detection
Glypican-2 levels in cerebrospinal fluid predict the status of adult hippocampal neurogenesis
Authors: S Lugert, T Kremer, R Jagasia, A Herrmann, S Aigner, C Giachino, I Mendez-Dav, AM Gardier, JP Carralot, H Meisterman, A Augustin, MD Saxe, J Lamerz, G Duran-Pach, A Ducret, V Taylor, DJ David, C Czech
Sci Rep, 2017-04-25;7(0):46543.
Species: Human
Sample Types: CSF
Applications: ELISA Development