Human GM130/GOLGA2 Antibody Summary
Met528-Gln606
Accession # Q08379
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human GM130/GOLGA2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and ZR-75 human breast cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8199) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GM130/GOLGA2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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GM130/GOLGA2 in HeLa Human Cell Line. GM130/GOLGA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8199) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi apparatus vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human GM130/GOLGA2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and ZR-75 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for GM130/GOLGA2 at approximately 141 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8199) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GM130/GOLGA2
GM130, also known as Golgin subfamily A member 2 and GOLGA2, is a 130 kDa member of the Golgin protein family. GM130/GOLGA2 is a component of the Golgi membrane involved in maintaining cis-Golgi structure. GM130/GOLGA2 protein is thought to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been predicted. Human GM130/GOLGA2 protein is 1002 amino acids (aa) in length, and over aa 528‑606, shares 90% sequence identity with its mouse and rat orthologs.
Product Datasheets
Citations for Human GM130/GOLGA2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
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The HSP GRP94 interacts with macrophage intracellular complement C3 and impacts M2 profile during ER stress
Authors: K Chaumonnot, S Masson, H Sikner, A Bouchard, V Baverel, PS Bellaye, B Collin, C Garrido, E Kohli
Cell Death & Disease, 2021-01-22;12(1):114.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Oncogenes overexpressed in metastatic oral cancers from patients with pain: potential pain mediators released in exosomes
Authors: A Bhattachar, MN Janal, R Veeramacha, I Dolgalev, Z Dubeykovsk, NH Tu, H Kim, S Zhang, AK Wu, M Hagiwara, AR Kerr, MD DeLacure, BL Schmidt, DG Albertson
Sci Rep, 2020-09-07;10(1):14724.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
SERINC5 Inhibits the Secretion of Complete and Genome-Free Hepatitis B Virions Through Interfering With the Glycosylation of the HBV Envelope
Authors: Y Liu, H Wang, J Zhang, J Yang, L Bai, B Zheng, T Zheng, Y Wang, J Li, W Zhang
Front Microbiol, 2020-04-30;11(0):697.
Species: Human
Sample Types: Whole Cell
Applications: ICC -
RAC1-Dependent ORAI1 Translocation to the Leading Edge Supports Lamellipodia Formation and Directional Persistence
Authors: AM Lopez-Guer, N Espinosa-B, I Sanchez-Lo, T Macartney, C Pascual-Ca, Y Orantos-Ag, L Rodriguez-, AB Perez-Oliv, V Mulero, E Pozo-Guisa, FJ Martin-Rom
Sci Rep, 2020-04-20;10(1):6580.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The potassium channel KCNJ13 is essential for smooth muscle cytoskeletal organization during mouse tracheal tubulogenesis
Authors: W Yin, HT Kim, S Wang, F Gunawan, L Wang, K Kishimoto, H Zhong, D Roman, J Preussner, S Guenther, V Graef, C Buettner, B Grohmann, M Looso, M Morimoto, G Mardon, S Offermanns, DYR Stainier
Nat Commun, 2018-07-19;9(1):2815.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Endosomal phosphatidylserine is critical for the YAP signalling pathway in proliferating cells
Authors: T Matsudaira, K Mukai, T Noguchi, J Hasegawa, T Hatta, SI Iemura, T Natsume, N Miyamura, H Nishina, J Nakayama, K Semba, T Tomita, S Murata, H Arai, T Taguchi
Nat Commun, 2017-11-01;8(1):1246.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
CLIPR-59: a protein essential for neuromuscular junction stability during mouse late embryonic development.
Authors: Couesnon A, Offner N, Bernard V, Chaverot N, Backer S, Dimitrov A, Perez F, Molgo J, Bloch-Gallego E
Development, 2013-04-01;140(7):1583-93.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC
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