Human Growth Hormone Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 201 | 595 | 1091 | 209 | 623 | 1152 |
Standard Deviation | 9.4 | 18.8 | 26 | 18.2 | 45.6 | 63.6 |
CV% | 4.7 | 3.2 | 2.4 | 8.7 | 7.3 | 5.5 |
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 197 | 586 | 1092 | 215 | 639 | 1189 |
Standard Deviation | 8 | 13.8 | 29.8 | 20.2 | 49.5 | 82.2 |
CV% | 4.1 | 2.4 | 2.7 | 9.4 | 7.7 | 6.9 |
Recovery
The recovery of Growth Hormone spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 100 | 97-105 |
EDTA Plasma (n=4) | 108 | 101-117 |
Heparin Plasma (n=4) | 107 | 99-117 |
Serum (n=4) | 105 | 98-111 |
Linearity
Scientific Data
Preparation and Storage
Background: Growth Hormone
Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretory granules.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Growth Hormone Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Magnetically actuating implantable pump for the on-demand and needle-free administration of human growth hormone
Authors: SH Lee, CR Kim, YC Cho, SN Kim, BH Kim, C Lee, HB Ji, JH Han, CG Park, H Hong, YB Choy
International journal of pharmaceutics, 2022-03-12;618(0):121664.
Species: Rat
Sample Types: Plasma
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Local non-pituitary growth hormone is induced with aging and facilitates epithelial damage
Authors: V Chesnokova, S Zonis, A Apostolou, HQ Estrada, S Knott, K Wawrowsky, K Michelsen, A Ben-Shlomo, R Barrett, V Gorbunova, K Karalis, S Melmed
Cell Reports, 2021-12-14;37(11):110068.
Species: Human
Sample Types: Cell Culture Supernates
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High-throughput mediation analysis of human proteome and metabolome identifies mediators of post-bariatric surgical diabetes control
Authors: JM Dreyfuss, Y Yuchi, X Dong, V Efthymiou, H Pan, DC Simonson, A Vernon, F Halperin, P Aryal, A Konkar, Y Sebastian, BW Higgs, J Grimsby, CM Rondinone, S Kasif, BB Kahn, K Foster, R Seeley, A Goldfine, V Djordjilov, ME Patti
Nature Communications, 2021-11-29;12(1):6951.
Species: Human
Sample Types: Plasma
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Structural and functional characterization of recombinant human growth hormone isolated from transgenic pig milk
Authors: SY Lee, JH Han, EK Lee, YK Kim, SA Hwang, SH Lee, M Kim, GY Cho, JH Hwang, SJ Kim, JG Yoo, SK Cho, KJ Lee, WK Cho
PLoS ONE, 2020-07-31;15(7):e0236788.
Species: Porcine
Sample Types: Cell Culture Supernates, Milk
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Investigating Stress Response during Vaginal Delivery and Elective Cesarean Section through Assessment of Levels of Cortisol, Interleukin 6 (IL-6), Growth Hormone (GH) and Insulin-Like Growth Factor 1 (IGF-1)
Authors: N Kiriakopou, S Grigoriadi, E Maziotis, A Philippou, A Rapani, P Giannelou, P Tsioulou, K Sfakianoud, A Kontogeorg, P Bakas, G Mastorakos, M Koutsilier, M Simopoulou
J Clin Med, 2019-07-27;8(8):.
Species: Human
Sample Types: Serum
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Metabolomics in plasma of Malawian children 7?years after surviving severe acute malnutrition: "ChroSAM" a cohort study
Authors: C Bourdon, N Lelijveld, D Thompson, PS Dalvi, GB Gonzales, D Wang, M Alipour, E Wine, E Chimwezi, JC Wells, M Kerac, R Bandsma, MJ Nyirenda
EBioMedicine, 2019-06-27;0(0):.
Species: Human
Sample Types: Plasma
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Implantable multireservoir device with stimulus-responsive membrane for on-demand and pulsatile delivery of growth hormone
Authors: SH Lee, H Piao, YC Cho, SN Kim, G Choi, CR Kim, HB Ji, CG Park, C Lee, CI Shin, WG Koh, YB Choy, JH Choy
Proc. Natl. Acad. Sci. U.S.A., 2019-05-23;0(0):.
Species: Rat
Sample Types: Plasma
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Relative Bioavailability of a Single 4-mg Dose of Somatropin Administered by Subcutaneous Injection or by Needle-free Device and Coadministered With the Growth Hormone Inhibitor Octreotide Acetate in Healthy Adult Subjects
Authors: DB Brimhall, N Petri, P D'Angelo
Clin Ther, 2018-04-24;0(0):.
Species: Human
Sample Types: Serum
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Elevations in growth hormone and glucagon-like peptide-2 levels on admission are associated with increased mortality in trauma patients.
Authors: Matthew P Rowan, Darrick J Beckman, Julie A Rizzo, Claire L Isbell, Christopher E White, Stephen M Cohn, Kevin K Chung
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, 2016-10-04;0(0):1757-7241.
Species: Human
Sample Types: Serum
Applications: ELISA Capture -
Endocrine responses during overnight recovery from exercise: impact of nutrition and relationships with muscle protein synthesis.
Authors: Betts JA, Beelen M, Stokes KA, Saris W HM, van Loon L JC
Int J Sport Nutr Exerc Metab, 2011-08-10;21(5):398-409.
Species: Human
Sample Types: Plasma
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Skeletal muscle myofibrillar and sarcoplasmic protein synthesis rates are affected differently by altitude-induced hypoxia in native lowlanders.
Authors: Holm L, Haslund ML, Robach P, van Hall G, Calbet JA, Saltin B, Lundby C
PLoS ONE, 2010-12-20;5(12):e15606.
Species: Human
Sample Types: Plasma
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CYP2C19 pharmacogenetics in advanced cancer: compromised function independent of genotype.
Authors: Helsby NA, Lo WY, Sharples K, Riley G, Murray M, Spells K, Dzhelai M, Simpson A, Findlay M
Br. J. Cancer, 2008-10-21;99(8):1251-5.
Species: Human
Sample Types: Serum
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