Human GRP75/HSPA9B Antibody

Catalog #: MAB35841 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB35841
MAB35841-SP

GRP75/HSPA9B in Human Fibroblast Menangioma.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GRP75/HSPA9B Antibody Summary

Species Reactivity

Human

Specificity

Detects human GRP75/HSPA9B in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 419605

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GRP75/HSPA9B
Glu542-Gln679
Accession # P38646

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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GRP75/HSPA9B in Human Fibroblast Menangioma. GRP75/HSPA9B was detected in immersion fixed paraffin-embedded sections of human fibroblast menangioma using 15 µg/mL Mouse Anti-Human GRP75/HSPA9B Monoclonal Antibody (Catalog # MAB35841) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

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GRP75/HSPA9B in Human Fibroblast Menangioma. GRP75/HSPA9B was detected in immersion fixed paraffin-embedded sections of human fibroblast menangioma using 25 µg/mL Mouse Anti-Human GRP75/HSPA9B Monoclonal Antibody (Catalog # MAB35841) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRP75/HSPA9B

The 70 kDa heat shock proteins (HSP70s) are a highly conserved family of stress response proteins. The HSP70 family of proteins contains both heat/stress inducible and constitutively expressed members known as heat shock cognate proteins. Glucose Regulated 75 kDa Protein (GRP75, also known as HSPA9B, mitochondrial HSP70, and mortalin-2) is a 679 amino acid (aa) heat shock cognate protein. Many HSPs function as molecular chaperones, facilitating the folding of other cellular proteins. GRP75 is a mitochondrial protein involved in protein translocation into the mitochondria. Proteins crossing the mitochondrial membrane require unfolding before entering translocation pores in the mitochondrial outer membrane. GRP75 together with other inner membrane proteins of the mitochondria mediate this process. GRP75 also plays a role in the control of cell cycle progression.

Long Name

75 KD Glucose Regulated Protein

Entrez Gene IDs

3313 (Human); 15527 (Mouse)

Alternate Names

75 kDa glucose-regulated protein; CSA; GRP75; GRP-75; GRP75mthsp75; Heat shock 70 kDa protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9 (mortalin); heat shock 70kDa protein 9B (mortalin-2); HSPA9; HSPA9B; HSPA9BMGC4500; Mortalin; mortalin, perinuclear; Mortalin-2; MOT; MOT2; mot-2; MTHSP75; p66-mortalin; PBP74; PBP74CSA; Peptide-binding protein 74; stress-70 protein, mitochondrial