Human HLA-DR Antibody

Detection of HLA-DRA in Human Tonsil via Multiplex Immunofluorescence staining on COMET™​ HLA-DRA was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human HLA-DRA Monoclonal Antibody (Catalog # MAB11555) at 1 µg/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​​

Detection of Human HLA‑DR by Western Blot. Western Blot shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human HLA‑DR Monoclonal Antibody (Catalog # MAB11555) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HLA‑DR at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of HLA‑DR in Human Tonsil. HLA‑DR was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human HLA‑DR Monoclonal Antibody (Catalog # MAB11555) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human HLA‑DR by Simple Western^TM. Simple Western shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, loaded at 1 mg/ml. A specific band was detected for HLA‑DR at approximately 42 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human HLA‑DR Monoclonal Antibody (Catalog # MAB11555). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.