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Human HM74A/PUMA-G/GPR109A Antibody

Catalog #: MAB2760
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2760
MAB2760-SP
Detection of HM74A/PUMA-G/GPR109A in Human Blood Neutrophils by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HM74A/PUMA-G/GPR109A Antibody Summary

Species Reactivity
Human
Specificity
Detects human HM74A/GPR109A. Stains human HM74A/GPR109A-transfected cells but not irrelevant transfectants. Cross-reactivity with HM74 (GPR109B) was not tested.
Source
Monoclonal Rat IgG2B Clone # 245106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human HM74A/GPR109A
Met1-Pro363
Accession # Q8TDS4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of HM74A/PUMA-G/GPR109A antibody in Human Blood Neutrophils antibody by Flow Cytometry. View Larger

Detection of HM74A/PUMA-G/GPR109A in Human Blood Neutrophils by Flow Cytometry. Human peripheral blood neutrophils were stained with (A) Rat Anti-Human HM74A/PUMA-G/GPR109A Monoclonal Antibody (Catalog # MAB2760) or (B) isotype control antibody (Catalog # MAB0061) followed by anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113) and Mouse anti-Human Fc gamma RIII (CD16) PE-conjugated Monoclonal Antibody (Catalog # FAB2546P. View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of HM74A/PUMA-G/GPR109A antibody in HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP antibody by Flow Cytometry. View Larger

Detection of HM74A/PUMA-G/GPR109A in HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human HM74A/PUMA-G/GPR109A or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Human HM74A/PUMA-G/GPR109A Monoclonal Antibody (Catalog # MAB2760) followed by anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HM74A/PUMA-G/GPR109A

HM74A, also known as GPR109A, is a high affinity G-protein coupled receptor for nicotinic acid. It is primarily expressed in adipose tissue and spleen.

Long Name
G Protein-coupled Receptor HM74A/G Protein-coupled Receptor 109A
Entrez Gene IDs
338442 (Human); 80885 (Mouse)
Alternate Names
GPR109A; HCA2; HCAR2; HM74A; hydroxycarboxylic acid receptor 2; Niacr1; Nicotinic Acid Receptor; PUMAG

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Citations for Human HM74A/PUMA-G/GPR109A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Activation of Gpr109a, receptor for niacin and the commensal metabolite butyrate, suppresses colonic inflammation and carcinogenesis.
    Authors: Singh N, Gurav A, Sivaprakasam S, Brady E, Padia R, Shi H, Thangaraju M, Prasad P, Manicassamy S, Munn D, Lee J, Offermanns S, Ganapathy V
    Immunity, 2014-01-09;40(1):128-39.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Neutrophil apoptosis mediated by nicotinic acid receptors (GPR109A).
    Authors: Kostylina G, Simon D, Fey MF, Yousefi S, Simon HU
    Cell Death Differ., 2007-10-12;15(1):134-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC

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