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Human HNF-3 beta /FoxA2 Antibody

Catalog #: AF2400
67 Citations 2 Reviews Datasheet / COA / SDS
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AF2400
AF2400-SP
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Detection of HNF‑3 beta /FoxA2-regulated Genes by Chromatin Immunoprecipitation.
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Citations (67)
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Human HNF-3 beta /FoxA2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HNF‑3 beta /FoxA2
Met242-Ser457
Accession # Q9Y261
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
HepG2 human hepatocellular carcinoma cell line
Immunohistochemistry
3-15 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed endoderm differentiated BG01V human embryonic stem cells and immersion fixed HepG2 Human Hepatocellular Carcinoma Cells (Positive) and absent in THP‑1 Human Acute Monocytic Leukemia Cells (Negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Chromatin Immunoprecipitation (ChIP) Detection of HNF-3 beta /FoxA2-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of HNF‑3 beta /FoxA2-regulated Genes by Chromatin Immunoprecipitation. Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF-3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human HNF-3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. TheE-RABPpromoter was detected by standard PCR.

Immunocytochemistry HNF-3 beta /FoxA2 antibody in Endoderm Differentiated BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

HNF‑3 beta /FoxA2 in Endoderm Differentiated BG01V Human Stem Cells. HNF-3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF-3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry HNF-3 beta /FoxA2 antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

HNF‑3 beta /FoxA2 in Human Liver. HNF-3 beta /FoxA2 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HNF-3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry View Larger

Detection of HNF‑3 beta /FoxA2 in HepG2 Cells (Positive) and THP‑1 Cells (Negative). HNF‑3 beta /FoxA2 was detected in immersion fixed HepG2 Human Hepatocellular Carcinoma Cells (Positive) and absent in THP‑1 Human Acute Monocytic Leukemia Cells (Negative) using Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot View Larger

Detection of Human HNF‑3 beta /FoxA2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HNF‑3 beta /FoxA2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HNF-3 beta/FoxA2

HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.

Long Name
Hepatocyte Nuclear Factor-3, beta
Entrez Gene IDs
3170 (Human)
Alternate Names
forkhead box A2; Forkhead box protein A2; FoxA2; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta; hepatocyte nuclear factor 3-beta; HNF3 beta; HNF-3 beta; HNF-3B; HNF-3-beta; HNF3BTCF-3B; MGC19807; TCF3B; Transcription factor 3B

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Citations for Human HNF-3 beta /FoxA2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

67 Citations: Showing 1 - 10
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  1. Reproducible generation of human liver organoids (HLOs) on a pillar plate platform via microarray 3D bioprinting
    Authors: Shrestha, S;Lekkala, VKR;Acharya, P;Kang, SY;Vanga, MG;Lee, MY;
    bioRxiv : the preprint server for biology
    Species: Human hepegivirus
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Post-translational proteomics platform identifies neurite outgrowth impairments in Parkinson's disease GBA-N370S dopamine neurons
    Authors: H Bogetofte, BJ Ryan, P Jensen, SI Schmidt, DLE Vergoossen, MB Barnkob, LN Kiani, U Chughtai, R Heon-Rober, MC Caiazza, W McGuinness, R Márquez-Gó, J Vowles, FS Bunn, J Brandes, P Kilfeather, JP Connor, HJR Fernandes, TM Caffrey, M Meyer, SA Cowley, MR Larsen, R Wade-Marti
    Cell Reports, 2023-03-03;42(3):112180.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. CRISPR/Cas9-mediated generation of hESC lines with homozygote and heterozygote p.R331W mutation in CTBP1 to model HADDTS syndrome
    Authors: EY Akda?, S Turan, D Guhathakur, A Ekici, S Salar, DC Lie, B Winner, A Fejtova
    Stem Cell Research, 2022-12-30;67(0):103012.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  4. Generation of an induced pluripotent stem cell line (IPCASi001-A) from an autism spectrum disorder individual without intellectual disability
    Authors: L Yang, S Lu, Z Yang, J Yao, P Zhou, M Zhao
    Stem Cell Research, 2022-12-05;66(0):102994.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. A reference human induced pluripotent stem cell line for large-scale collaborative studies
    Authors: CB Pantazis, A Yang, E Lara, JA McDonough, C Blauwendra, L Peng, H Oguro, J Kanaujiya, J Zou, D Sebesta, G Pratt, E Cross, J Blockwick, P Buxton, L Kinner-Bib, C Medura, C Tompkins, S Hughes, M Santiana, F Faghri, MA Nalls, D Vitale, S Ballard, YA Qi, DM Ramos, KM Anderson, J Stadler, P Narayan, J Papademetr, L Reilly, MP Nelson, S Aggarwal, LU Rosen, P Kirwan, V Pisupati, SL Coon, SW Scholz, T Priebe, M Öttl, J Dong, M Meijer, LJM Janssen, VS Lourenco, R van der Ka, D Crusius, D Paquet, AC Raulin, G Bu, A Held, BJ Wainger, RMC Gabriele, JM Casey, S Wray, D Abu-Bonsra, CL Parish, MS Beccari, DW Cleveland, E Li, IVL Rose, M Kampmann, C Calatayud, P Verstreken, L Heinrich, MY Chen, B Schüle, D Dou, ELF Holzbaur, MC Zanellati, R Basundra, M Deshmukh, S Cohen, R Khanna, M Raman, ZS Nevin, M Matia, J Van Lent, V Timmerman, BR Conklin, K Johnson Ch, K Zhang, S Funes, DA Bosco, L Erlebach, M Welzer, D Kronenberg, G Lyu, E Arenas, E Coccia, L Sarrafha, T Ahfeldt, JC Marioni, WC Skarnes, MR Cookson, ME Ward, FT Merkle
    Cell Stem Cell, 2022-12-01;29(12):1685-1702.e22.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: ICC
  6. Analysis of Influenza A virus infection in human induced pluripotent stem cells (hiPSCs) and their derivatives
    Authors: K Ruangrung, W Chakritbud, S Thongon, S Rungarunle, M Wattanapan, C Boonarkart, O Suptawiwat, N Sirinontha, DR Smith, P Auewarakul
    Virus research, 2022-11-19;323(0):199009.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Induced Pluripotent Stem Cell (iPSC) Lines from a Family with Resistant Epileptic Encephalopathy Caused by Compound Heterozygous Mutations in SZT2 Gene
    Authors: C Cattelani, I Battistell, F Di Leva, G Fioravanti, F Benedicent, F Stanzial, C Schwienbac, F Fanelli, PP Pramstalle, AA Hicks, L Conti, C Corti
    International Journal of Molecular Sciences, 2022-10-28;23(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Transplantation of a human induced pluripotent stem cell-derived airway epithelial cell sheet into the middle ear of rats
    Authors: T Tada, H Ohnishi, N Yamamoto, F Kuwata, Y Hayashi, H Okuyama, T Morino, Y Kasai, H Kojima, K Omori
    Regenerative Therapy, 2022-01-14;19(0):77-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants
    Authors: P Aldrin-Kir, M Åkerblom, T Cardoso, S Nolbrant, AF Adler, X Liu, A Heuer, M Davidsson, M Parmar, T Björklund
    Stem Cell Reports, 2021-12-30;17(1):159-172.
    Species: Human, Rat, Transgenic Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  10. Sequence logic at enhancers governs a dual mechanism of endodermal organ fate induction by FOXA pioneer factors
    Authors: RJ Geusz, A Wang, DK Lam, NK Vinckier, KD Alysandrat, DA Roberts, J Wang, S Kefalopoul, A Ramirez, Y Qiu, J Chiou, KJ Gaulton, B Ren, DN Kotton, M Sander
    Nature Communications, 2021-11-17;12(1):6636.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ChIP, IHC
  11. Functional human iPSC-derived alveolar-like cells cultured in a miniaturized 96?Transwell air-liquid interface model
    Authors: T Bluhmki, S Traub, AK Müller, S Bitzer, E Schruf, MT Bammert, M Leist, F Gantner, JP Garnett, R Heilker
    Scientific Reports, 2021-08-23;11(1):17028.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  12. Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels
    Authors: A Fütterer, A Talavera-G, T Pons, J de Celis, J Gutiérrez, V Domínguez, C Martínez-A
    Cell Death & Disease, 2021-06-21;12(7):637.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  13. SARS-CoV-2 Infection Causes Dopaminergic Neuron Senescence
    Authors: S Chen, Y Han, L Yang, T Kim, M Nair, O Harschnitz, P Wang, J Zhu, SY Koo, X Tang, L Lacko, V Chandar, Y Bram, T Zhang, W Zhang, F He, J Caicedo, Y Huang, T Evans, P van der Va, MJ Titulaer, JKH Spoor, RL Furler, P Canoll, J Goldman, S Przedborsk, R Schwartz, D Ho, L Studer
    Research square, 2021-05-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  14. Airway basal stem cells generate distinct subpopulations of PNECs
    Authors: H Mou, Y Yang, MA Riehs, J Barrios, M Shivaraju, AL Haber, DT Montoro, K Gilmore, EA Haas, B Paunovic, J Rajagopal, SO Vargas, RL Haynes, A Fine, WV Cardoso, X Ai
    Cell Reports, 2021-04-20;35(3):109011.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  15. Human naive epiblast cells possess unrestricted lineage potential
    Authors: G Guo, GG Stirparo, SE Strawbridg, D Spindlow, J Yang, J Clarke, A Dattani, A Yanagida, MA Li, S Myers, BN Özel, J Nichols, A Smith
    Cell Stem Cell, 2021-04-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Autologous transplant therapy alleviates motor and depressive behaviors in parkinsonian monkeys
    Authors: Y Tao, SC Vermilyea, M Zammit, J Lu, M Olsen, JM Metzger, L Yao, Y Chen, S Phillips, JE Holden, V Bondarenko, WF Block, TE Barnhart, N Schultz-Da, K Brunner, H Simmons, BT Christian, ME Emborg, SC Zhang
    Nature Medicine, 2021-03-01;0(0):.
    Species: Primate - Rhesus macaque
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  17. Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01
    Authors: J Piao, S Zabierowsk, BN Dubose, EJ Hill, M Navare, N Claros, S Rosen, K Ramnarine, C Horn, C Fredrickso, K Wong, B Safford, S Kriks, A El Maarouf, U Rutishause, C Henchcliff, Y Wang, I Riviere, S Mann, V Bermudez, S Irion, L Studer, M Tomishima, V Tabar
    Cell Stem Cell, 2021-02-04;28(2):217-229.e7.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC
  18. Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use
    Authors: TW Kim, J Piao, SY Koo, S Kriks, SY Chung, D Betel, ND Socci, SJ Choi, S Zabierowsk, BN Dubose, EJ Hill, EV Mosharov, S Irion, MJ Tomishima, V Tabar, L Studer
    Cell Stem Cell, 2021-02-04;28(2):343-355.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Derivation of induced pluripotent stem cells (iPSCs) by retroviral transduction of skin fibroblasts from four patients suffering 7q11.23 microduplication syndrome
    Authors: B Kuebler, B Aran, R Flores, LA Pérez-Jura, A Veiga, I Cuscó, R Corominas
    Stem Cell Res, 2020-11-19;49(0):102092.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Generation of induced pluripotent stem cells (iPSCs) by retroviral transduction of skin fibroblasts from four patients suffering Williams-Beuren syndrome (7q11.23 deletion)
    Authors: B Kuebler, B Aran, R Flores, LA Pérez-Jura, A Veiga, R Corominas, I Cuscó
    Stem Cell Research, 2020-11-16;49(0):102087.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Generation, establishment and characterization of a pluripotent stem cell line (CVTTHi001-A) from primary fibroblasts isolated from a patient with activated PI3 kinase delta syndrome (APDS2)
    Authors: M Inglés-Fer, M Martin-Ina, L Herrera, M Villaverde, S Santos, MA Vesga, E Garreta, I Martín-Rui, AM Aransay, J Anguita, B Barreña, LM Allende, LI Gonzalez-G, C Eguizabal
    Stem Cell Res, 2020-11-07;49(0):102082.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Direct On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells
    Authors: E Naumovska, G Aalderink, C Wong Valen, K Kosim, A Nicolas, S Brown, P Vulto, KS Erdmann, D Kurek
    Int J Mol Sci, 2020-07-14;21(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. HDAC3-dependent transcriptional repression of FOXA2 regulates FTO/m6A/MYC signaling to contribute to the development of gastric cancer
    Authors: Z Yang, X Jiang, Z Zhang, Z Zhao, W Xing, Y Liu, X Jiang, H Zhao
    Cancer Gene Ther., 2020-07-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  24. A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
    Authors: L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen
    Cell Stem Cell, 2020-06-19;27(1):125-136.e7.
    Species: Human
    Sample Types: Organoids, Whole Cells
    Applications: ICC, IHC
  25. Generation of intestinal organoids derived from human pluripotent stem cells for drug testing
    Authors: S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi
    Sci Rep, 2020-04-06;10(1):5989.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Effects of Natural Progesterone and Synthetic Progestin on Germ Layer Gene Expression in a Human Embryoid Body Model
    Authors: YY Kim, H Kim, CS Suh, HC Liu, Z Rosenwaks, SY Ku
    Int J Mol Sci, 2020-01-24;21(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. Hepatic TET3 contributes to type-2 diabetes by inducing the HNF4alpha fetal isoform
    Authors: Da Li, T Cao, X Sun, S Jin, Di Xie, X Huang, X Yang, GG Carmichael, HS Taylor, S Diano, Y Huang
    Nat Commun, 2020-01-17;11(1):342.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  28. Generation of three induced pluripotent cell lines (iPSCs) from an Aicardi-Gouti�res syndrome (AGS) patient harboring a deletion in the genomic locus of the sterile alpha motif and HD domain containing protein 1 (SAMHD1)
    Authors: NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, L Wendeburg, D Steinemann, C Elpers, F Rutsch, R König
    Stem Cell Res, 2020-01-09;43(0):101697.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. Hepatic TET3 contributes to type-2 diabetes by inducing the HNF4alpha fetal isoform
    Authors: Da Li, T Cao, X Sun, S Jin, Di Xie, X Huang, X Yang, GG Carmichael, HS Taylor, S Diano, Y Huang
    Nat Commun, 2020;11(1):342.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  30. A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
    Authors: L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen
    Cell Stem Cell, 2020;27(1):125-136.e7.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  31. Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency
    Authors: N Bredenkamp, J Yang, J Clarke, GG Stirparo, F von Meyenn, S Dietmann, D Baker, R Drummond, Y Ren, D Li, C Wu, M Rostovskay, S Eminli-Mei, A Smith, G Guo
    Stem Cell Reports, 2019-11-07;13(6):1083-1098.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons
    Authors: M Riessland, B Kolisnyk, TW Kim, J Cheng, J Ni, JA Pearson, EJ Park, K Dam, D Acehan, LS Ramos-Espi, W Wang, J Zhang, JW Shim, G Ciceri, L Brichta, L Studer, P Greengard
    Cell Stem Cell, 2019-09-19;25(4):514-530.e8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation
    Authors: K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu
    Cell Rep, 2019-07-09;28(2):382-393.e7.
    Species: Human
    Sample Types: Cell Lysates, Chromatin
    Applications: Immunoprecipitation, Western Blot
  34. Impact of Sleep-Wake-Associated Neuromodulators and Repetitive Low-Frequency Stimulation on Human iPSC-Derived Neurons
    Authors: R Yokoi, M Okabe, N Matsuda, A Odawara, A Karashima, I Suzuki
    Front Neurosci, 2019-05-29;13(0):554.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  35. CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons
    Authors: C Calatayud, G Carola, I Fernández-, M Valtorta, S Jiménez-De, M Díaz, J Soriano-Fr, G Cappellett, J García-San, Á Raya, A Consiglio
    Sci Rep, 2019-05-02;9(1):6811.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  36. A hPSC-based platform to discover gene-environment interactions that impact human ?-cell and dopamine neuron survival
    Authors: T Zhou, TW Kim, CN Chong, L Tan, S Amin, Z Sadat Badi, S Mukherjee, Z Ghazizadeh, H Zeng, M Guo, M Crespo, T Zhang, R Kenyon, CL Robinson, E Apostolou, H Wang, JZ Xiang, T Evans, L Studer, S Chen
    Nat Commun, 2018-11-16;9(1):4815.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  37. Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors
    Authors: H Ida, T Akiyama, K Ishiguro, SK Goparaju, Y Nakatake, N Chikazawa-, S Sato, H Kimura, Y Yokoyama, M Nagino, MSH Ko, SBH Ko
    Stem Cell Res Ther, 2018-10-25;9(1):277.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  38. AAVvector-mediated in vivo reprogramming into pluripotency
    Authors: E Senís, L Mosteiro, S Wilkening, E Wiedtke, A Nowrouzi, S Afzal, R Fronza, H Landerer, M Abad, D Niopek, M Schmidt, M Serrano, D Grimm
    Nat Commun, 2018-07-09;9(1):2651.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  39. Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS)
    Authors: B Kuebler, B Aran, L Miquel-Ser, Y Muñoz, E Ars, G Bullich, M Furlano, R Torra, M Marti, A Veiga, A Raya
    Stem Cell Res, 2017-09-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  40. CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells
    Authors: KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima
    Stem Cell Reports, 2017-06-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  41. Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines
    Authors: L Miquel-Ser, A Duarri, Y Muñoz, B Kuebler, B Aran, C Costa, M Martí, M Comabella, S Malhotra, X Montalban, A Veiga, A Raya
    Stem Cell Res, 2017-06-07;24(0):155-159.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  42. Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells
    Authors: Y Kondo, T Toyoda, R Ito, M Funato, Y Hosokawa, S Matsui, T Sudo, M Nakamura, C Okada, X Zhuang, A Watanabe, A Ohta, N Inagaki, K Osafune
    Diabetologia, 2017-05-22;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: ICC
  43. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
    Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
    Cell Rep, 2017-04-04;19(1):20-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  44. Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells
    Authors: F Sampazioti, MC de Brito, I Geti, A Bertero, NR Hannan, L Vallier
    Nat Protoc, 2017-03-23;12(4):814-827.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  45. Generation of thalamic neurons from mouse embryonic stem cells
    Authors: A Shiraishi, K Muguruma, Y Sasai
    Development, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  46. Triple Staining Including FOXA2 Identifies Stem Cell Lineages Undergoing Hepatic and Biliary Differentiation in Cirrhotic Human Liver
    Authors: Leslie E Rogler
    J. Histochem. Cytochem., 2016-11-24;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: ICC
  47. Cell Expansion During Directed Differentiation Of Stem Cells Toward The Hepatic Lineage
    Stem Cells Dev., 2016-11-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  48. Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
    Sci Rep, 2016-08-10;6(0):31063.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  49. A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells.
    Authors: Tsankov A, Akopian V, Pop R, Chetty S, Gifford C, Daheron L, Tsankova N, Meissner A
    Nat Biotechnol, 2015-10-26;33(11):1182-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  50. Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.
    Authors: Villa-Diaz L, Kim J, Lahann J, Krebsbach P
    Stem Cells Transl Med, 2014-10-13;3(12):1410-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  51. Physiological characterisation of human iPS-derived dopaminergic neurons.
    Authors: Hartfield, Elizabet, Yamasaki-Mann, Michiko, Ribeiro Fernandes, Hugo J, Vowles, Jane, James, William, Cowley, Sally A, Wade-Martins, Richard
    PLoS ONE, 2014-02-21;9(2):e87388.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  52. Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, oct4 and sox2.
    Authors: Montserrat N, Ramirez-Bajo MJ, Xia Y, Sancho-Martinez I, Moya-Rull D, Miquel-Serra L, Yang S, Nivet E, Cortina C, Gonzalez F, Izpisua Belmonte JC, Campistol JM
    J. Biol. Chem., 2012-05-21;287(29):24131-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  53. Treatment with small molecules is an important milestone towards the induction of pluripotency in neural stem cells derived from human cord blood.
    Authors: Szablowska-Gadomska, Ilona, Sypecka, Joanna, Zayat, Valery, Podobinska, Martyna, Pastwinska, Anna, Pienkowska-Grela, Barbara, Buzanska, Leonora
    Acta Neurobiol Exp (Wars), 2012-01-01;72(4):337-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  54. Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
    Authors: Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L
    Cell Transplant, 2011-12-21;21(6):1321-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  55. Complete meiosis from human induced pluripotent stem cells.
    Authors: Eguizabal C, Montserrat N, Vassena R, Barragan M, Garreta E, Garcia-Quevedo L, Vidal F, Giorgetti A, Veiga A, Belmonte JC
    Stem Cells, 2011-08-01;29(8):1186-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  56. A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3.
    Authors: Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ
    J. Cell. Sci., 2011-05-24;124(0):1992-2000.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  57. CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells.
    Authors: Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H
    Stem Cells, 2011-04-01;29(4):609-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  58. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
    Authors: Klim JR, Li L, Wrighton PJ
    Nat. Methods, 2010-11-14;7(12):989-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  59. Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm.
    Authors: Chayosumrit M, Tuch B, Sidhu K
    Biomaterials, 2009-10-14;31(3):505-14.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  60. Linking the p53 tumour suppressor pathway to somatic cell reprogramming.
    Authors: Kawamura T, Suzuki J, Wang YV, Menendez S, Morera LB, Raya A, Wahl GM, Belmonte JC
    Nature, 2009-08-09;460(7259):1140-4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  61. Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells.
    Authors: Raya A, Rodriguez-Piza I, Guenechea G, Vassena R, Navarro S, Barrero MJ, Consiglio A, Castella M, Río P, Sleep E, Gonzalez F, Tiscornia G, Garreta E, Aasen T, Veiga A, Verma IM, Surralles J, Bueren J, Belmonte JC
    Nature, 2009-05-31;460(7251):53-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  62. Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells.
    Authors: Zhang D, Jiang W, Liu M, Sui X, Yin X, Chen S, Shi Y, Deng H
    Cell Res., 2009-04-01;19(4):429-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  63. Generation of pluripotent stem cells from adult human testis.
    Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
    Nature, 2008-10-08;456(7220):344-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  64. Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium.
    Authors: Nakanishi M, Kurisaki A, Hayashi Y, Warashina M, Ishiura S, Kusuda-Furue M, Asashima M
    FASEB J., 2008-09-22;23(1):114-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  65. Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells.
    Authors: Zhang P, Li J, Tan Z, Wang C, Liu T, Chen L, Yong J, Jiang W, Sun X, DU L, Ding M, Deng H
    Blood, 2007-11-27;111(4):1933-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  66. Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure.
    Authors: Cho CH, Parashurama N, Park EY, Suganuma K, Nahmias Y, Park J, Tilles AW, Berthiaume F, Yarmush ML
    FASEB J., 2007-10-17;22(3):898-909.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  67. Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions.
    Authors: Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S
    Proc. Natl. Acad. Sci. U.S.A., 2006-04-21;103(18):6907-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human HNF-3 beta /FoxA2 Antibody
By Anonymous on 09/10/2021
Application: IHC Sample Tested: Prostate cancer tissue Species: Human
Immunohistochemistry Human HNF-3 beta /FoxA2 Antibody AF2400
Human HNF-3 beta /FoxA2 Antibody
By Anonymous on 02/15/2019
Application: ICC/IF Sample Tested: alveoli Species: Human

dapi- BLUE
magenta - foxa2

Immunocytochemistry/Immunofluorescence Human HNF-3 beta /FoxA2 Antibody AF2400