Human ICAM-3/CD50 Antibody

Catalog #: AF715 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF715
AF715-SP

All ICAM-3/CD50 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ICAM-3/CD50 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ICAM‑3/CD50 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) ICAM-1 is observed and less than 1% cross-reactivity with recombinant mouse (rm) ICAM-1, recombinant rat ICAM-1, rhICAM-2, rhVCAM-1, and rmVCAM-1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ICAM‑3/CD50

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ICAM‑3/CD50 Fc Chimera (Catalog # 715-IC)

Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood monocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-3/CD50

Intercellular Adhesion Molecule‑3 (ICAM‑3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA‑1 (CD11a/CD18) and alpha _d/ beta ₂. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM‑3 has not been identified in the mouse.

References