Human ICAM-3/CD50 Biotinylated Antibody

Catalog #: BAF813
1 Citation Datasheet / COA / SDS

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BAF813

All ICAM-3/CD50 Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ICAM-3/CD50 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ICAM‑3/CD50 in ELISAs and Western blots. In Western blot, approximately 2% cross‑reactivity with recombinant human PECAM is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ICAM‑3/CD50
Gln30-His485
Accession # CAA49473

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ICAM‑3/CD50 Fc Chimera (Catalog # 715-IC)

Human ICAM-3/CD50 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human ICAM‑3/CD50 Antibody (Catalog # BBA15)

Standard: Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF (Catalog # 715-IC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-3/CD50

Intercellular Adhesion Molecule-3 (ICAM-3; also known as CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha _d/ beta ₂. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.

References

Hayflick, J. et al. (1998) Immunologic Res. 17:313.

Long Name

Intercellular Adhesion Molecule 3

Entrez Gene IDs

3385 (Human)

Alternate Names

CD50 antigen; CD50; CDW50CDw50; ICAM3; ICAM-3; ICAM-Rintercellular adhesion molecule-3; intercellular adhesion molecule 3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human ICAM-3/CD50 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Soluble adhesion molecules in juvenile rheumatoid arthritis.
Authors: Bloom BJ, Miller LC, Tucker LB, Schaller JG, Blier PR
J. Rheumatol., 1999-09-01;26(9):2044-8.
Species: Human
Sample Types: Serum
Applications: ELISA Development