Human ICAT Antibody

Catalog #: MAB3016
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3016-100
MAB3016-SP

ICAT in Human Fibrocystic Breast Tissue.

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All ICAT Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ICAT Antibody Summary

Species Reactivity

Human

Specificity

Detects human ICAT in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 536017

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human ICAT
Met1-Gln81
Accession # Q9NSA3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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ICAT in Human Fibrocystic Breast Tissue. ICAT was detected in immersion fixed paraffin-embedded sections of human fibrocystic breast tissue using Mouse Anti-Human ICAT Monoclonal Antibody (Catalog # MAB3016) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAT

Human ICAT is a physiological inhibitor of the beta -Catenin/Wnt signaling pathway. It is 9 kDa in size and found in both cytosol and nucleus. The molecule contains an alpha ‑helical N-terminal region (aa 9‑54) and an acidic, extended C-terminal region (aa 55‑78). The alpha -helical region binds to beta -Catenin, displacing CBP/p300, while the acidic C-terminus interacts with the beta -Catenin N-terminus, blocking Tcf interaction. Human ICAT shares 97% aa sequence identity with mouse and bovine ICAT, respectively.

Long Name

Beta-Catenin-Interacting Protein

Entrez Gene IDs

56998 (Human)

Alternate Names

beta-catenin-interacting protein ICAT; catenin, beta interacting protein 1; CTNNBIP1; ICAT; ICATbeta-interacting protein 1; Inhibitor of beta-catenin and Tcf-4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human ICAT Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

miR-26b-5p/TCF-4 Controls the Adipogenic Differentiation of Human Adipose-derived Mesenchymal Stem Cells
Authors: Y Luo, H Ji, Y Cao, X Ding, M Li, H Song, S Li, C WaTableng, H Wu, J Meng, H Du
Cell Transplant, 2020-01-01;29(0):9636897209344.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot