Human ICOS Antibody Summary
Glu21-Phe141
Accession # Q9Y6W8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of ICOS in HEK293 Human Cell Line Transfected with Human ICOS by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human ICOS was stained with Mouse Anti-Human ICOS Monoclonal Antibody (Catalog # MAB69753, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Human B7-H2 Binding to ICOS-transfected HEK293 Human Cell Line is Blocked by Human ICOS Antibody. In a functional Flow Cytometry test, biotinylated recombinant Human B7-H2 (Catalog # 8206-B7, 5 ng/mL) binds to Human ICOS-transfected HEK293 cells (black dotted line). Binding is completely blocked (filled histogram) by 2.5 µg/mL of Mouse Anti-Human ICOS Monoclonal Antibody (Catalog # MAB69753). Mouse IgG3 isotype (Catalog # MAB007) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Sterptavidin-APC (Catalog # F0050).
ICOS binding to B7-H2 blocked by Human ICOS Antibody. In a Surface Plasmon Resonance test, Recombinant Human B7-H2 (Catalog # 8206-B7) coated at 30 ug/mL binds to Recombinant Human B7-H7 (Catalog # 8084-B7, 25 µg/mL (green line). Binding is completely blocked (orange line) by 50 µg/mL of Mouse Anti-Human ICOS Monoclonal Antibody (Catalog # MAB69753). Non-blocking anti-ICOS antibody (Clone 669244) was used as control and did not affect ICOS binding to B7-H2 (blue line).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICOS
Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
- Aicher, A. et al. (2000) J. Immunol. 164:4689.
- Coyle, A.J. et al. (2000) Immunity 13:95.
- Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
- Hutloff, A. et al. (1999) Nature 397:263.
- Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
Product Datasheets
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