Human IFN-alpha / beta R1 PE-conjugated Antibody

Catalog #: FAB245P
5 Citations Datasheet / COA / SDS

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FAB245P

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Detection of IFN‑ alpha / beta R1 in U937 Human cell line by Flow Cytometry.

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Citations (5)

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Human IFN-alpha / beta R1 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IFN-alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) IFN‑ gamma R1, rhIFN-gamma R2, recombinant mouse IFN-alpha / beta R2, rhIL-10 R beta, rhIL-10 R alpha, or rhIL-20 R beta.

Source

Monoclonal Mouse IgG₁ Clone # 85228

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta R1
Lys28-Lys436
Accession # AAA52730

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IFN‑ alpha / beta R1 in U937 Human cell line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human IFN-a/ beta R1 PE-conjugated Monoclonal Antibody (Catalog # FAB245P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IFN-alpha/beta R1

Type I interferons (IFN-alpha, IFN-beta, IFN-omega ) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.

Long Name

Interferon alpha/beta Receptor 1

Entrez Gene IDs

3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)

Alternate Names

alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1

Product Datasheets

Product Datasheet

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Product Specific Notices

Sold under license from Pestka Biomedical Laboratories, Inc. d/b/a PBL Interferon Source.

Related Research Areas

Citations for Human IFN-alpha / beta R1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Sex differences in neutrophil biology modulate response to type I interferons and immunometabolism
Authors: S Gupta, S Nakabo, LP Blanco, LJ O'Neil, G Wigerblad, RR Goel, P Mistry, K Jiang, C Carmona-Ri, DW Chan, X Wang, HL Pedersen, M Gadkari, KN Howe, F Naz, S Dell'Orso, SA Hasni, C Dempsey, A Buscetta, PA Frischmeye, P Kruszka, M Muenke, LM Franco, HW Sun, MJ Kaplan
Proc. Natl. Acad. Sci. U.S.A., 2020-06-29;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Symmetric Arginine Dimethylation Is Selectively Required for mRNA Splicing and the Initiation of Type I and Type III Interferon Signaling
Authors: PJ Metz, KA Ching, T Xie, P Delgado Cu, S Niessen, JH Tatlock, K Jensen-Per, BW Murray
Cell Rep, 2020-02-11;30(6):1935-1950.e8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Safe and effective treatment of experimental neuroblastoma and glioblastoma using systemically administered triple microRNA-detargeted oncolytic Semliki Forest virus
Clin Cancer Res, 2016-09-16;0(0):.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Type I interferon (IFN-alpha/beta) rescues B-lymphocytes from apoptosis via PI3Kdelta/Akt, Rho-A, NFkappaB and Bcl-2/Bcl(XL).
Authors: Badr G, Saad H, Waly H
Cell. Immunol., 2010-02-24;263(1):31-40.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Enhancement of cell type specificity by quantitative modulation of a chimeric ligand.
Authors: Cironi P, Swinburne IA, Silver PA
J. Biol. Chem., 2008-01-29;283(13):8469-76.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry