Human IFN-gamma Biotinylated Antibody

Catalog #: BAF285
12 Citations Datasheet / COA / SDS

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BAF285

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All IFN-gamma Antibodies

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Citations (12)

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Human IFN-gamma Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross-reactivity with recombinant rhesus macaque IFN‑ gamma is observed and less than 0.05% cross-reactivity with recombinat rat IFN-gamma, recombinant feline IFN-gamma, recombinant equine IFN-gamma, recombinant cotton rat IFN-gamma, recombinant canine IFN-gamma, recombinant porcine IFN-gamma, and recombinant mouse IFN-gamma is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF)
Met1-Gln144
Accession # Q14609

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IFN-gamma (Catalog # 285-IF)

Immunocytochemistry

5-15 µg/mL

See below

Human IFN-gamma Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human IFN‑ gamma Antibody (Catalog # MAB2852)

Standard: Recombinant Human IFN-gamma Protein (Catalog # 285-IF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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IFN‑ gamma in Human PBMCs. IFN‑ gamma was detected in immersion fixed PMA- and ionomycin-stimulated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human IFN‑ gamma Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF285) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry

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IFN‑ gamma in Human PBMCs. IFN-gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA, ionomyocin, and monensin using Human IFN-gamma Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF285) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Long Name

Interferon gamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Alternate Names

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human IFN-gamma Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Defining T cell receptor repertoires using nanovial-based binding and functional screening
Authors: Koo, D;Mao, Z;Dimatteo, R;Noguchi, M;Tsubamoto, N;McLaughlin, J;Tran, W;Lee, S;Cheng, D;de Rutte, J;Burton Sojo, G;Witte, ON;Di Carlo, D;
Proceedings of the National Academy of Sciences of the United States of America
Species: Human hepegivirus
Sample Types: Cell Culture Supernates
Applications: Capture Assay
Immunogenic epitope panel for accurate detection of non-cross-reactive T cell response to SARS-CoV-2
Authors: A Titov, R Shaykhutdi, OV Shcherbako, YV Serdyuk, SA Sheetikov, KV Zornikova, AV Maleeva, A Khmelevska, DV Dianov, NT Shakirova, DB Malko, M Shkurnikov, S Nersisyan, A Tonevitsky, E Khamaganov, AV Ershov, EY Osipova, RV Nikolaev, DE Pershin, VA Vedmedskia, M Maschan, VR Ginanova, GA Efimov
JCI Insight, 2022-05-09;7(9):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Detection
Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum
Authors: A Stumpf, T Brandstett, J Hübner, J Rühe
PLoS ONE, 2019-12-02;14(12):e0225525.
Species: Human
Sample Types: Serum
Applications: ELISA Detection
Decreased allergy incidence in children supplemented with E. coli O83:K24:H31 and its possible modes of action
Authors: J Hrdý, K Vlasáková, V ?erný, L Súkeníková, O Novotná, P Petrásková, K Boráková, R Lodinová-Ž, L Kolá?ová, L Prokešová
Eur. J. Immunol., 2018-10-21;0(0):.
Species: Human
Sample Types: Plasma
Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
PLoS ONE, 2017-04-14;12(4):e0175712.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
Clin. Chem., 2007-11-01;53(11):2010-2.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007-10-22;329(1):214-8.
Species: Human
Sample Types: Serum
Applications: Luminex Development
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood, 2007-02-08;109(10):4272-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clin. Immunol., 2006-06-16;120(2):220-31.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation.
Authors: Deiva K, Geeraerts T, Salim H, Leclerc P, Hery C, Hugel B, Freyssinet JM, Tardieu M
Eur. J. Neurosci., 2004-12-01;20(12):3222-32.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr

Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Authors: Copeland S, Siddiqui J, Remick D
J. Immunol. Methods, 2004-01-01;284(1):99-106.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Array Development