Human IGFBP-L1 Biotinylated Antibody

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BAF3877
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Human IGFBP-L1 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGFBP-L1 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) IGFBP-L1 is observed and less than 1% cross-reactivity with recombinant human (rh) IGFBP-2, -3, -4, -5, -6, -7, rmIGFBP-1, and rmIGFBP-rP10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP-L1
Ser25-Met278
Accession # Q8WX77
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IGFBP-L1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-L1

IGFBP-L1 is a secreted 38 kDa protein that shares 43% amino acid sequence identity with IGFBP-7 (IGFBP-rP1, Mac25). Although it was also referred to as IGFBP‑rP4 in one publication, it is distinct from Cyr61, a CCN family protein also named IGFBP-rP4 at one time. IGFBP-L1 contains an N-terminal IGFBP motif, a Kazal‑type serine protease inhibitor region and a C-terminal Ig-like domain. Human and mouse mature IGFBP-L1 share 76% amino acid sequence homology.

Long Name
Insulin-like Growth Factor Binding Protein-like 1
Entrez Gene IDs
347252 (Human); 75426 (Mouse)
Alternate Names
bA113O24.1; IGFBPL1 insulin-like growth factor binding protein-like 1; IGFBPL1; IGFBP-L1; IGFBP-RP4

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