Human IGSF4C/SynCAM4 Antibody

Catalog #: MAB4164 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4164
MAB4164-SP

Detection of IGSF4C/SynCAM4 in A172 Human Cell Line by Flow Cytometry.

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Human IGSF4C/SynCAM4 Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human (rh) IGSF4C/SynCAM4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) IGSF4A, rhIGSF4B, rhIGSF4D, or rhIGSF8 is observed.

Source

Monoclonal Rat IgG1 Clone # 404619

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IGSF4C/SynCAM4 (Catalog # 4164-S4) under non-reducing conditions only

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

Immersion fixed WM‑115 human malignant melanoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IGSF4C/SynCAM4 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Rat Anti-Human IGSF4C/SynCAM4 Monoclonal Antibody (Catalog # MAB4164, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113).

Immunocytochemistry

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IGSF4C/SynCAM4 in WM‑115 Human Cell Line. IGSF4C/SynCAM4 was detected in immersion fixed WM‑115 human malignant melanoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Rat Anti-Human IGSF4C/SynCAM4 Monoclonal Antibody (Catalog # MAB4164) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IGSF4C/SynCAM4

IGSF4C is an immunoglobulin superfamily member that is also known as Nectin-like protein 4 (Necl-4), synaptic cell adhesion molecule 4 (SynCAM4), or tumor suppressor in lung cancer-like 2 (TSLL-2) (1, 2). The four IGSF4 proteins, designated A, B, C and D, are type I transmembrane glycoproteins expressed mainly in neurons, but also in lung, kidney, bladder, prostate and testis (1‑5). Their extracellular domains (ECD) share 35‑50% amino acid (aa) identity and each contain one V‑type Ig-like and two C2-type Ig-like domains. These domains are responsible for Ca⁺⁺‑independent homophilic and heterophilic interactions. The 388 aa human IGSF4C contains a 20 aa signal sequence, a 304 aa ECD that shares 98‑99% amino acid identity with mouse, rat, canine and bovine IGSF4C, a 21 aa transmembrane sequence, and a 43 aa cytoplasmic domain. The apparent size of mouse or human IGSF4C may be variably reported as 48‑67 kDa, probably due to differences in glycosylation (2, 5, 8). In the peripheral nervous system, IGSF4C is expressed on Schwann cells, and its internodal interaction with IGSF4A (Necl-1, SynCAM-3) on axons is critical for adhesion and myelination (6‑8). In the brain, all IGSF4 family members are expressed at high levels concurrent with synapse formation (4). In the cerebellum, IGSF4C is expressed on Purkinje cells, with complementary expression of IGSF4 on granule cells (4). Heterophilic interaction with IGSF4D (Necl-3, SynCAM2) has also been identified, but homophilic interaction is unlikely (4, 6). IGSF4C is also proposed as a tumor suppressor that is downregulated in many prostate cancers and gliomas (1, 5).

References

Fukuhara, H. et al. (2001) Oncogene 20:5401.
Biederer, T. et al. (2006) Genomics 87:139.
Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
Thomas, L. A. et al. (2008) J. Comp. Neurol. 510:47.
Williams, Y. N. et al. (2005) Oncogene 25:1446.
Fogel, A. I. et al. (2007) J. Neurosci. 27:12516.
Spiegel, I. et al. (2007) Nat. Neurosci. 10:861.
Maurel, P. et al. (2007) J. Cell Biol. 178:861.

Long Name

Immunoglobulin Superfamily, Member 4B

Entrez Gene IDs

199731 (Human); 260299 (Mouse)

Alternate Names

CADM4; cell adhesion molecule 4; IGSF4C; IGSF4Cnectin-like 4; Immunoglobulin superfamily member 4C; immunoglobulin superfamily, member 4C; Necl-4; NECL4synCAM4; Nectin-like protein 4; SynCAM4; TSLC1-like 2; TSLC1-like protein 2; TSLL2; TSLL2SynCAM4