Human IL-1 RI APC-conjugated Antibody

Catalog #: FAB269A
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB269A-100
FAB269A-025

Detection of IL‑1 RI in HUVEC Human Cells by Flow Cytometry.

1 Image

All IL-1 RI Antibodies

Product Details

Citations (5)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-1 RI APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-1 RI in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RI

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of IL‑1 RI in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human IL-1 RI APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB269A, filled histogram) or control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-1 RI

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

References

Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

Long Name

Interleukin 1 Receptor I

Entrez Gene IDs

3554 (Human); 16177 (Mouse)

Alternate Names

CD121 antigen-like family member A; CD121a antigen; CD121a; IL-1 RI; IL1R; IL1R1; IL1RI; IL-1RI; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-1 RI APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
Nature Immunology, 2022-12-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Single-cell sequencing of human white adipose tissue identifies new cell states in health and obesity
Authors: AD Hildreth, F Ma, YY Wong, R Sun, M Pellegrini, TE O'Sullivan
Nature Immunology, 2021-04-27;22(5):639-653.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Differential role of Interleukin-1 and Interleukin-6 in K-Ras-driven pancreatic carcinoma undergoing mesenchymal transition
Authors: I Siddiqui, M Erreni, MA Kamal, C Porta, F Marchesi, S Pesce, F Pasqualini, S Schiarea, C Chiabrando, A Mantovani, P Allavena
Oncoimmunology, 2017-11-01;7(2):e1388485.
Species: Human
Sample Types: Whole Cell
Applications: Flow Cytometry
Oncogenic Transformation Can Orchestrate Immune Evasion and Inflammation in Human Mesenchymal Stem Cells Independently of Extrinsic Immune-Selective Pressure.
Authors: Miranda A, Funes J, Sanchez N, Limia C, Mesa M, Quezada S, Perez R, de Leon J
Cancer Res, 2015-06-11;75(15):3032-42.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
The IL18RAP region disease polymorphism decreases IL-18RAP/IL-18R1/IL-1R1 expression and signaling through innate receptor-initiated pathways.
Authors: Hedl M, Zheng S, Abraham C
J Immunol, 2014-05-19;192(12):5924-32.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry