Human IL-1 RII PE-conjugated Antibody Summary
Phe14-Glu343 (Ser56Gly and Glu297Gly)
Accession # P27930
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑1 RII in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with either (A) Mouse Anti-Human IL-1 RII PE-conjugated Monoclonal Antibody (Catalog # FAB663P) or (B) Mouse IgG1Phycoerythrin Isotype Control (IC002P) and Mouse anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A). View our protocol for Staining Membrane-associated Proteins.
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Detection of IL‑1 RII in Human PBMCs stimulated to induce Tregs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs), stimulated to induce Regulatory T Cells (Tregs), were treated with 10 ug/mL Anti-CD3, 5 ug/mL Anti-CD28, 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 48 hours and stained with Rat Anti-Human LRRC32/GARP APC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Human IL-1 RII PE-conjugated Monoclonal Antibody (Catalog # FAB663P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). Cells were gated on CD4. View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-1 RII
Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex. An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta, but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.
Product Datasheets
Citations for Human IL-1 RII PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Phenotypic changes of peripheral blood mononuclear cells upon corticosteroid treatment in idiopathic intermediate uveitis
Authors: Karoline Walscheid
Clin. Immunol, 2016-10-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
J Immunol, 2015-08-14;195(6):2818-28.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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This antibody was used to stain human peripheral blood for analysis by flow cytometry. Eosinophils were identified from CD45+ leukocytes and IL-1RII expression was measured in the PE channel. This antibody generates consistent fluorescence which is maintained after fixation. Can be used for various cell types.
