Human IL-10 Antibody Summary
Ser19-Asn178
Accession # P22301
Applications
Human IL-10 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2172) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10
Interleukin-10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
Product Datasheets
Citations for Human IL-10 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Dual-Enhanced Plasmonic Biosensing for Point-of-Care Sepsis Detection
Authors: Lip Ket Chin, Jun-Yeong Yang, Benjamin Chousterman, Sunghoon Jung, Do-Geun Kim, Dong-Ho Kim et al.
ACS Nano
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Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum
Authors: A Stumpf, T Brandstett, J Hübner, J Rühe
PLoS ONE, 2019-12-02;14(12):e0225525.
Species: Human
Sample Types: Serum
Applications: ELISA Capture -
Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
PLoS ONE, 2017-04-14;12(4):e0175712.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture) -
Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips
Authors: Manfè Valentina, Fleckner Jan, Nørby Lisby Peder, Zhang Bo, Dai Hongjie, Keller Pernille
BMC Biotechnology
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IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
Immunol. Lett., 2009-11-04;127(2):150-6.
Species: Human
Sample Types: Cell Culture Supernates, Whole Tissue
Applications: ELISA Development, IHC-P -
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS, 2007;21(10):1263-72.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Selective regulation of IL-10 signaling and function by zymosan.
Authors: Du Z, Kelly E, Mecklenbrauker I, Agle L, Herrero C, Paik P, Ivashkiv LB
J. Immunol., 2006-04-15;176(8):4785-92.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Authors: Copeland S, Siddiqui J, Remick D
J. Immunol. Methods, 2004-01-01;284(1):99-106.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Array Development
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