Human IL-12 R beta 2 Antibody

Catalog #: MAB19591 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB19591
MAB19591-SP

Detection of Human IL‑12 R beta 2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-12 R beta 2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-12 R beta 2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL‑12 R beta 1 or recombinant mouse IL-12 R beta 1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 622509

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 2
Lys24-Cys622
Accession # Q99665

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL‑12 R beta 2 by Western Blot. Western blot shows lysates of human CD4^{+in vitrodifferentiated Th1 cells. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human IL-12 R beta 2 Monoclonal Antibody (Catalog # MAB19591) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-12 R beta 2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.}

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12 R beta 2

IL-12 R beta 2 is a type I cytokine receptor family protein that associates with IL 12 R beta 1 to form a functional high affinity receptor complex for IL-12. The extracellular region of IL 12 R beta 2 contains five fibronectin type 3 domains. The cytoplasmic region is alternatively spliced, giving rise to two isoforms. IL-12 R beta 2 is up‑regulated in Th1 cells during inflammatory responses. The extracellular regions of human and mouse IL‑12 R beta 2 share 69% amino acid sequence identity.

Long Name

Interleukin 12 Receptor beta 2

Entrez Gene IDs

3595 (Human); 16162 (Mouse)

Alternate Names

IL-12 R beta 2; IL-12 receptor beta 2; IL-12 receptor subunit beta-2; IL12R beta 2; IL-12R subunit beta-2; IL12RB2; IL-12Rb2; IL-12R-beta-2; interleukin 12 receptor, beta 2; interleukin-12 receptor beta-2 chain; interleukin-12 receptor subunit beta-2