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Human IL-13 Quantikine ELISA Kit

Catalog #: D1300B
31 Citations 1 Review Datasheet / COA / SDS
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D1300B
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QC20: Quantikine Immunoassay Control Group 3
Human IL-13 Simple Plex Assay Linearity
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Citations (31)
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Reviews (1)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-13 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
57.4 pg/mL
Assay Range
125.0 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-13
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human IL-13 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-13 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-13 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-13 accurately. Results obtained using natural human IL-13 showed dose response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-13.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 360 1031 2002 319 932 1895
Standard Deviation 12.9 27.5 43.2 22.2 52.6 108
CV% 3.6 2.7 2.2 7 5.6 5.7

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 352 960 1919 365 1087 2199
Standard Deviation 11.1 21.3 33.3 25.8 63.1 105
CV% 3.2 2.2 1.7 7.1 5.8 4.8

Recovery

The recovery of IL-13 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 108 100-120
EDTA Plasma (n=4) 106 98-120
Heparin Plasma (n=4) 104 94-120
Serum (n=4) 102 95-113

Linearity

To assess linearity of the assay, samples were spiked with high concentrations of IL-13 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-13 Simple Plex Assay Linearity
Human IL-13 ELISA Linearity

Scientific Data

N/A Human IL-13 ELISA Calibrator Diluent RD5R Standard Curve View Larger

Human IL-13 ELISA Calibrator Diluent RD5R Standard Curve

N/A Human IL-13 ELISA Calibrator Diluent RD6-64 Standard Curve View Larger

Human IL-13 ELISA Calibrator Diluent RD6-64 Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-13

IL-13 (Interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 R alpha 2 binds IL-13 and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex. IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis.

Long Name:
Interleukin 13
Entrez Gene IDs:
3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
Alternate Names:
ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-13 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Gene expression profiles and bioinformatics analysis in lung samples from ovalbumin-induced asthmatic mice
    Authors: Y Song, J Jiang, Q Bai, S Liu, Y Zhang, C Xu, H Piao, L Li, G Yan
    BMC pulmonary medicine, 2023-02-02;23(1):50.
    Species: Mouse
    Sample Types: BALF
  2. The IL-4/IL-13 signaling axis promotes prostatic fibrosis
    Authors: Q D'Arcy, M Gharaee-Ke, A Zhilin-Rot, JA Macoska
    PLoS ONE, 2022-10-06;17(10):e0275064.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Immunoregulatory effects of Lactococcus lactis-derived extracellular vesicles in allergic asthma
    Authors: DH Lee, HK Park, HR Lee, H Sohn, S Sim, HJ Park, YS Shin, YK Kim, Y Choi, HS Park
    Clinical and translational allergy, 2022-03-01;12(3):e12138.
    Species: Human
    Sample Types: Serum
  4. Interleukin-31 promotes fibrosis and T helper 2 polarization in systemic sclerosis
    Authors: A Kuzumi, A Yoshizaki, KM Matsuda, H Kotani, Y Norimatsu, M Fukayama, S Ebata, T Fukasawa, A Yoshizaki-, Y Asano, K Morikawa, Y Kazoe, K Mawatari, T Kitamori, S Sato
    Nature Communications, 2021-10-12;12(1):5947.
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates, Serum, Tissue Homogenates
  5. Socioeconomic status, financial stress, and glucocorticoid resistance among youth with asthma: Testing the moderation effects of maternal involvement and warmth
    Authors: Y Jiang, AK Farrell, ET Tobin, HE Mair-Meije, DE Wildman, F Luca, RB Slatcher, S Zilioli
    Brain, Behavior, and Immunity, 2021-05-17;0(0):.
    Species: Human
    Sample Types: Plasma
  6. Cytokine Profiles of Non-Small Cell Lung Cancer Patients Treated with Concurrent Chemoradiotherapy with Regards to Radiation Pneumonitis Severity
    Authors: BK Jeong, JH Kim, MH Jung, KM Kang, YH Lee
    Journal of Clinical Medicine, 2021-02-11;10(4):.
    Species: Human
    Sample Types: Plasma
  7. Clinical characteristics of patients with not well-controlled severe asthma in Japan: Analysis of the Keio Severe Asthma Research Program in Japanese population (KEIO-SARP) registry
    Authors: T Tanosaki, H Kabata, M Matsusaka, J Miyata, K Masaki, T Mochimaru, S Okuzumi, M Kuwae, R Watanabe, Y Suzuki, K Sayama, K Izuhara, K Asano, K Fukunaga
    Allergol Int, 2020-07-08;0(0):.
    Species: Human
    Sample Types: Serum
  8. Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis
    Authors: T Auguet, L Bertran, J Binetti, C Aguilar, S Martínez, F Sabench, JM Lopez-Dupl, JA Porras, D Riesco, D Del Castil, C Richart
    Int J Mol Sci, 2020-06-11;21(11):.
    Species: Human
    Sample Types: Plasma
  9. ALDH3A1 Overexpression in Melanoma and Lung Tumors Drives Cancer Stem Cell Expansion, Impairing Immune Surveillance through Enhanced PD-L1 Output
    Authors: E Terzuoli, C Bellan, S Aversa, V Ciccone, L Morbidelli, A Giachetti, S Donnini, M Ziche
    Cancers (Basel), 2019-12-06;11(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Identification of sarilumab pharmacodynamic and predictive markers in patients with inadequate response to TNF inhibition: a biomarker substudy of the phase 3 TARGET study
    Authors: C Gabay, J Msihid, M Zilberstei, C Paccard, Y Lin, NMH Graham, A Boyapati
    RMD Open, 2018-03-14;4(1):e000607.
    Species: Human
    Sample Types: Serum
  11. The JAK2 pathway is activated in idiopathic pulmonary fibrosis
    Authors: J Milara, G Hernandez, B Ballester, A Morell, I Roger, P Montero, J Escrivá, JM Lloris, M Molina-Mol, E Morcillo, J Cortijo
    Respir. Res., 2018-02-06;19(1):24.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Up-regulation of serum periostin and squamous cell carcinoma antigen levels in infants with acute bronchitis due to respiratory syncytial virus
    Authors: H Nakamura, K Akashi, M Watanabe, S Ohta, J Ono, Y Azuma, N Ogasawara, K Yamamoto, N Shimizu, H Tsutsumi, K Izuhara, T Katsunuma
    Allergol Int, 2017-11-06;0(0):.
    Species: Human
    Sample Types: Serum
  13. IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
    Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
    J Transl Med, 2015-12-16;13(0):381.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Absence of a role for interleukin-13 in inflammatory bowel disease.
    Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T
    Eur J Immunol, 2014-02-01;44(2):370-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
    Authors: Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H
    Arthritis Res. Ther., 2011-03-24;13(2):R52.
    Species: Human
    Sample Types: Serum
  16. Thymic stromal lymphopoietin-induced human asthmatic airway epithelial cell proliferation through an IL-13-dependent pathway.
    Authors: Semlali A, Jacques E, Koussih L, Gounni AS, Chakir J
    J. Allergy Clin. Immunol., 2010-03-17;125(4):844-50.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Mast cell-dependent contraction of human airway smooth muscle cell-containing collagen gels: influence of cytokines, matrix metalloproteases, and serine proteases.
    Authors: Margulis A, Nocka KH, Brennan AM, Deng B, Fleming M, Goldman SJ, Kasaian MT
    J. Immunol., 2009-07-10;183(3):1739-50.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2008-12-03;123(1):59-66.e4.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
    Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
    Int. Immunol., 2008-08-13;20(10):1321-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Detection of pancreatic cancer using antibody microarray-based serum protein profiling.
    Authors: Ingvarsson J, Wingren C, Carlsson A, Ellmark P, Wahren B, Engstrom G, Harmenberg U, Krogh M, Peterson C, Borrebaeck CA
    Proteomics, 2008-06-01;8(11):2211-9.
    Species: Human
    Sample Types: Serum
  21. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008-04-01;38(4):1012-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
    Authors: Miller R, Sadhukhan R, Wu C
    J. Immunol. Methods, 2008-03-07;334(1):134-41.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. IL-13 involvement in eosinophilic esophagitis: transcriptome analysis and reversibility with glucocorticoids.
    Authors: Blanchard C, Mingler MK, Vicario M, Abonia JP, Wu YY, Lu TX, Collins MH, Putnam PE, Wells SI, Rothenberg ME
    J. Allergy Clin. Immunol., 2007-12-01;120(6):1292-300.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
    Authors: Lin YL, Liang YC, Chiang BL
    J. Leukoc. Biol., 2007-08-30;82(6):1473-80.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. A network-based analysis of the late-phase reaction of the skin.
    Authors: Benson M, Langston MA, Adner M, Andersson B, Torinssson-Naluai A, Cardell LO
    J. Allergy Clin. Immunol., 2006-05-11;118(1):220-5.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis.
    Authors: Hartl D, Latzin P, Zissel G, Krane M, Krauss-Etschmann S, Griese M
    Am. J. Respir. Crit. Care Med., 2006-03-16;173(12):1370-6.
    Species: Human
    Sample Types: Serum
  27. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
    Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
    Clin. Cancer Res., 2006-01-15;12(2):376-82.
    Species: Human
    Sample Types: Plasma
  28. Relationship between cytokines and the embryotoxicity of hydrosalpingeal fluid.
    Authors: Bedaiwy MA, Falcone T, Goldberg JM, Attaran M, Sharma R, Miller K, Nelson DR, Agarwal A
    J. Assist. Reprod. Genet., 2005-04-01;22(4):161-5.
    Species: Human
    Sample Types: Hypersalpingeal Fluid (HSF)
  29. Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas.
    Authors: Ripley D, Shoup B, Majewski A
    Gynecol. Oncol., 2004-03-01;92(3):761-8.
    Species: Human
    Sample Types: Tissue Homogenates
  30. Implications of serum TNF-beta and IL-13 in the treatment response of childhood nephrotic syndrome.
    Authors: Tain YL, Chen TY, Yang KD
    Cytokine, 2003-02-07;21(3):155-9.
    Species: Human
    Sample Types: Serum
  31. Modulation of human immune response by Echinococcus granulosus antigen B and its possible role in evading host defenses.
    Authors: Rigano R, Profumo E, Bruschi F, Carulli G, Azzara A, Ioppolo S, Buttari B, Ortona E, Margutti P, Teggi A, Siracusano A
    Infect. Immun., 2001-01-01;69(1):288-96.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human IL-13 Quantikine ELISA Kit
By Alisha Freeman on 11/27/2017
Sample Tested: A375 human melanoma cell line