Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody Summary
Glu20-Arg329
Accession # Q13478
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑18 R alpha /IL‑1 R5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PHA (5 µg/ml) and Recombinant Human IL-2 (Catalog # 202-IL;10 ng/ml) for 2 days were stained with Mouse Anti-Human IL-18 Ra/IL-1 R5 APC-conjugated Monoclonal Antibody (Catalog # FAB840A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-18 R alpha/IL-1 R5
Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also known as IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also known as IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.
- Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
- Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
- Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Product Datasheets
Citations for Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Altered expression of IL-18 binding protein and IL-18 receptor in basophils and mast cells of asthma patients
Authors: Z Wang, Z Liu, L Wang, J Wang, L Chen, H Xie, H Zhang, S He
Scand. J. Immunol., 2018-04-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R
Authors: H Zhang, J Wang, L Wang, H Xie, L Chen, S He
J. Cell. Mol. Med., 2017-09-18;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Enhanced Expression of IL-18 and IL-18BP in Plasma of Patients with Eczema: Altered Expression of IL-18BP and IL-18 Receptor on Mast Cells
Authors: Y Hu, J Wang, H Zhang, H Xie, W Song, Q Jiang, N Zhao, S He
Mediators Inflamm., 2017-08-03;2017(0):3090782.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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Detection of IL18Rα/IL-1R5 expression in lymphocytes and monocytes by flow cytometry: Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll gradient method from freshly drawn blood and stained for IL18Ralpha using the R&D mouse Anti-human IL-18alpha/IL-1R5-APC antibody (cat# FAB840A). As suggested by the manufacturer, 10ul of antibody was used per 10e6 cells. After gating on CD3+ lymphocytes (Fig.1a) and CD14+ monocytes (Fig.1b), IL-18Ralpha expression on T lymphocytes and monocytes were analyzed. Panel 1 shows Fluorescence Minus One (FMO) which served as negative control; panel 2 shows IL18alpha+ cells; panel 3 shows overlay of panel 1 and panel 2.
