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Human IL-2 Antibody

Catalog #: MAB202
17 Citations 2 Reviews Datasheet / COA / SDS
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MAB202-SP
MAB202-100
MAB202-500
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Cell Proliferation Induced by IL‑2 and Neutralization by Human IL‑2 Antibody.
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Citations (17)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.
Source
Monoclonal Mouse IgG1 Clone # 5334
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL‑2
Ala21-Thr153
Accession # NP_000577
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells treated with PMA and Ca2+ ionomycin, fixed with paraformaldehyde, and permeabilized with saponin
Neutralization
Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND50) is typically 0.015-0.03 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑2 and Neutralization by Human IL‑2 Antibody. View Larger

Cell Proliferation Induced by IL‑2 and Neutralization by Human IL‑2 Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202). The ND50 is typically 0.015-0.03 µg/mL.

Immunocytochemistry IL-2 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑2 in Human PBMCs. IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin 2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.

Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

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Citations for Human IL-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. CXCL13-producing CD4+ T cells accumulate in early phase of tertiary lymphoid structures in ovarian cancer
    Authors: M Ukita, J Hamanishi, H Yoshitomi, K Yamanoi, S Takamatsu, A Ueda, H Suzuki, Y Hosoe, Y Furutake, M Taki, K Abiko, K Yamaguchi, H Nakai, T Baba, N Matsumura, A Yoshizawa, H Ueno, M Mandai
    JCI Insight, 2022-06-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Immunogenicity profile in African green monkeys of a vaccine candidate based on a mutated form of human Interleukin-15
    Authors: Y Rodríguez-, LG Batista-Ro, A Llopiz-Arz, P Puente-Pér, R Martínez-C, J Castro-Vel, A Santos-Sav
    BMC immunology, 2021-12-18;22(1):79.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. An IL-2-grafted antibody immunotherapy with potent efficacy against metastatic cancer
    Authors: D Sahin, N Arenas-Ram, M Rath, U Karakus, M Hümbelin, M van Gogh, L Borsig, O Boyman
    Nature Communications, 2020-12-22;11(1):6440.
    Species: Human, Mouse
    Sample Types: Recombinant Protein, Serum
    Applications: ELISA Detection
  4. Suppressor of cytokine signaling 3 is crucial for interleukin-7 receptor re-expression after T-cell activation and interleukin-7 dependent proliferation
    Authors: A Güler, ML Venegas, E Adankwah, E Mayatepek, N Nausch, M Jacobsen
    Eur. J. Immunol., 2019-11-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Effects of pepsin and pepstatin on reflux tonsil hypertrophy in vitro
    Authors: JH Kim, SJ Jang, JW Yun, MH Jung, SH Woo
    PLoS ONE, 2018-11-08;13(11):e0207090.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Blocking IL-2 Signal In Vivo with an IL-2 Antagonist Reduces Tumor Growth through the Control of Regulatory T Cells
    Authors: T Carmenate, Y Ortíz, M Enamorado, K García-Mar, J Avellanet, E Moreno, L Graça, K León
    J. Immunol., 2018-04-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. IL-23 and IL-1? Drive Human Th17 Cell Differentiation and Metabolic Reprogramming in Absence of CD28 Costimulation
    Authors: S Revu, J Wu, M Henkel, N Rittenhous, A Menk, GM Delgoffe, AC Poholek, MJ McGeachy
    Cell Rep, 2018-03-06;22(10):2642-2653.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
    Sci Rep, 2016-09-02;6(0):32320.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  9. Activin A programs the differentiation of human TFH cells
    Nat Immunol, 2016-07-04;17(8):976-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
    Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
    J Biol Chem, 2015-07-16;290(37):22338-51.
    Species: Human
    Sample Types: Protein
    Applications: Neutralization
  11. Interleukin-2 alters distribution of CD144 (VE-cadherin) in endothelial cells.
    Authors: Kim, Dae Won, Zloza, Andrew, Broucek, Joseph, Schenkel, Jason M, Ruby, Carl, Samaha, Georges, Kaufman, Howard L
    J Transl Med, 2014-05-06;12(0):113.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
    Blood, 2012-05-10;119(26):6268-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Adenovirus-specific human T cells are pervasive, polyfunctional, and cross-reactive.
    Authors: Hutnick NA, Carnathan D, Demers K, Makedonas G, Ertl HC, Betts MR
    Vaccine, 2010-02-23;28(8):1932-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
    Authors: Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC
    Blood, 2007-02-13;109(11):4856-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Primary cutaneous T-cell lymphomas show a deletion or translocation affecting NAV3, the human UNC-53 homologue.
    Authors: Karenko L, Hahtola S, Paivinen S, Karhu R, Syrja S, Kahkonen M, Nedoszytko B, Kytölä S, Zhou Y, Blazevic V, Pesonen M, Nevala H, Nupponen N, Sihto H, Krebs I, Poustka A, Roszkiewicz J, Saksela K, Peterson P, Visakorpi T, Ranki A
    Cancer Res., 2005-09-15;65(18):8101-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  16. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004-06-01;112(2):274-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Reprogramming of IL-10 activity and signaling by IFN-gamma.
    Authors: Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB
    J. Immunol., 2003-11-15;171(10):5034-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

FAQs

  1. Can Catalog # MAB202 (anti-Human IL-2 antibody) be used for neutralization of mouse IL-2?

    • No. MAB202 is not cross-reactive to mouse IL-2 and so cannot be used for neutralization of mouse IL-2.

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Reviews for Human IL-2 Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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By Anonymous on 11/16/2020
Application: Flow Sample Tested: BUD-8 human fibroblast cell line Species: Human
Human IL-2 Antibody
By Anonymous on 12/12/2019
Application: WB Sample Tested: Serum Species: Human
Western Blot Human IL-2 Antibody MAB202