Human IL-21R Antibody

Catalog #: AF991
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF991
AF991-SP

All IL-21R Antibodies

Product Details

Citations (6)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-21R Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑21 R Subunit in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse IL‑21 R is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑21 R Subunit
Cys20-Pro236
Accession # Q9HBE5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL‑21 R Subunit Fc Chimera (Catalog # 991-R2)

Flow Cytometry

2.5 µg/10⁶ cells

Human whole blood CD19⁺ B cells

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-21R

Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta, IL-4 R alpha and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells and cell lines of T, B and NK lineage. The common gamma chain ( gamma _c) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

References

Parrish-Novak, et al. (2000) Nature 408:57.
Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
Asao, H. et al. (2001) J. Immunol. 167:1.

Long Name

Interleukin 21 Receptor

Entrez Gene IDs

50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)

Alternate Names

CD360 antigen; CD360; IL-21 R; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-21R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients.
Authors: Kaltenmeier C, Gawanbacht A, Beyer T, Lindner S, Trzaska T, van der Merwe J, Harter G, Gruner B, Fabricius D, Lotfi R, Schwarz K, Schutz C, Honig M, Schulz A, Kern P, Bommer M, Schrezenmeier H, Kirchhoff F, Jahrsdorfer B
J Immunol, 2015-03-16;194(8):3768-77.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay
Heterogeneous expression and function of IL-21R and susceptibility to IL-21-mediated apoptosis in follicular lymphoma cells.
Authors: de Totero D, Capaia M, Fabbi M, Croce M, Meazza R, Cutrona G, Zupo S, Loiacono F, Truini M, Ferrarini M, Ferrini S
Exp. Hematol., 2010-03-01;38(5):373-83.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Dissociation of its opposing immunologic effects is critical for the optimization of antitumor CD8+ T-cell responses induced by interleukin 21.
Authors: Ansen S, Butler MO, Berezovskaya A, Murray AP, Stevenson K, Nadler LM, Hirano N
Clin. Cancer Res., 2008-10-01;14(19):6125-36.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Interleukin-21: an interleukin-2 dependent player in rejection processes.
Authors: Baan CC, Balk AH, Dijke IE, Korevaar SS, Peeters AM, de Kuiper RP, Klepper M, Zondervan PE, Maat LA, Weimar W
Transplantation, 2007-06-15;83(11):1485-92.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
J. Immunol., 2007-05-01;178(9):5957-65.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Expression of interleukin-21 receptor, but not interleukin-21, in synovial fibroblasts and synovial macrophages of patients with rheumatoid arthritis.
Authors: Jungel A, Distler JH, Kurowska-Stolarska M, Seemayer CA, Seibl R, Forster A, Michel BA, Gay RE, Emmrich F, Gay S, Distler O
Arthritis Rheum., 2004-05-01;50(5):1468-76.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-Fr, Western Blot