Human IL-28 R alpha /IFN-lambda R1 Antibody

Catalog #: MAB5260 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5260
MAB5260-SP

Detection of IL‑28 R alpha /IFN‑ lambda R1 in Daudi Human Cell Line by Flow Cytometry.

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All IL-28 R alpha/IFN-lambda R1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-28 R alpha /IFN-lambda R1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-28 R alpha /IFN-lambda R1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-28 alpha is observed.

Source

Monoclonal Mouse IgG₁ Clone # 601106

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-28 R alpha /IFN-lambda R1
Arg21-Ala228
Accession # Q8IU57

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑28 R alpha /IFN‑ lambda R1 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-28 Ra/IFN-lambda R1 Monoclonal Antibody (Catalog # MAB5260, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Flow Cytometry

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Detection of IL‑28 R alpha /IFN‑ lambda R1 in PBMCs by Flow Cytometry. Peripheral blood mononuclear cells (PBMCs) either unstimulated (A) or treated (B) with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and Mouse Anti-Human IL-28 Ra/IFN-lambda R1 Monoclonal Antibody (Catalog # MAB5260) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-28 R alpha/IFN-lambda R1

IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a widely-expressed type I transmembrane glycoprotein that is cytokine receptor family 2 member 12 (CRF2-12). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R. Its ligands, IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and IL-29 (IFN‑ lambda 1), are mainly produced by antigen presenting cells in response to viruses. The 520 amino acid (aa) mouse IL-28 R alpha contains a 208 aa extracellular domain (ECD) with a fibronectin type III motif. The human IL-28 R alpha ECD shares ~67% aa identity with rat and human IL‑28 R alpha. Isoforms of 491, 437, 322, 283, 244, 211 and 184 aa have alternate N- or C-termini, or lack transmembrane or internal sequences.

Long Name

Interleukin 28 Receptor alpha

Entrez Gene IDs

163702 (Human); 242700 (Mouse); 362628 (Rat)

Alternate Names

class II cytokine receptor CRF2/12; CRF2/12; CRF2-12; Cytokine receptor class-II member 12; Cytokine receptor family 2 member 12; IFN-lambda R1; IFN-lambda receptor 1; IFN-lambda-R1; IFNLR; IFNLR1; IL-28 R alpha; IL-28 receptor subunit alpha; IL28R alpha; IL-28R1; IL28RA; IL-28Ra; IL-28R-alpha; Interferon lambda receptor 1; interferon lambda, receptor 1; interleukin 28 receptor A; interleukin 28 receptor, alpha (interferon, lambda receptor); interleukin 28 receptor, alpha; interleukin or cytokine receptor 2; interleukin-28 receptor subunit alpha; LICR2; Likely interleukin or cytokine receptor 2