Human IL-32 Biotinylated Antibody

Catalog #: BAF3040
4 Citations Datasheet / COA / SDS

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BAF3040

All IL-32 Antibodies

Product Details

Citations (4)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-32 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-32 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IL‑32 alpha
Cys2-Lys131
Accession # NP_001012651

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL‑32

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-32

Interleukin 32 (IL-32) is an N-glycosylated cytokine that is upregulated by inflammatory stimulation in monocytes, NK cells, epithelial cells, and pancreatic myofibroblasts (1-5). It cooperates with these stimuli to promote the expression of other proinflammatory molecules such as TNF-alpha, IL-6, IL-1 beta, IL-1 alpha, and CXCL8/IL‑8 (5-7). The longest of several IL-32 splicing variants is the 20-25 kDa gamma isoform which is also known as natural killer cell transcript 4 (NK4) (8, 9). The alpha isoform (IL-32 alpha ) lacks a portion of the putative signal peptide as well as 57 aa from the C-terminal region. IL-32 alpha is less potent than IL-32 beta, gamma, or δ at inducing the expression of proinflammatory molecules in peripheral blood mononuclear cells (PBMC) (8, 10). Neutrophil-derived Proteinase 3 (PR3) cleaves IL-32 alpha between Thr57 and Val58, a cleavage site that is retained in other IL-32 isoforms (11). The N-terminal fragment of PR3-cleaved IL-32 alpha shows increased potency at inducing CXCL2/MIP-2 and CXCL8 expression in PBMC relative to uncleaved IL-32 alpha (11, 12). IL-32 is highly expressed by colonic epithelial cells in inflammatory bowel disease and Crohn’s disease, rheumatoid arthritis synovium, and ductal epithelial cells in chronic pancreatitis and pancreatic cancer (5, 13-15). IL-32 inhibits HIV-1 replication in vitro, and it is elevated in the serum of HIV-1 patients (16, 17).

References

Netea, M.G. et al. (2006) PloS Med. 3:e277.
Nold-Petry, C.A. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3883.
Li, W. et al. (2009) Eur. J. Immunol. 39:1019.
Nishida, A. et al. (2008) Am. J. Physiol. Gastrointest. Liver Physiol. 294:G831.
Shoda, H. et al. (2006) Arthritis Res. Ther. 8:R166.
Netea, M.G. et al. (2005) Proc. Natl. Acad. Sci. USA 102:16309.
Hong, J. et al. (2010) Cytokine 49:171.
Kim, S-H. et al. (2005) Immunity 22:131.
Dahl, C.A. et al. (1992) J. Immunol. 148:597.
Choi, J-D. et al. (2009) Immunology 126:535.
Novick, D. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3316.
Kim, S. et al. (2008) BMB Rep. 41:814.
Shioya, M. et al. (2007) Clin. Exp. Immunol. 149:480.
Joosten, L.A.B. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3298.
Nishida, A. et al. (2009) J. Biol. Chem. 284:17868.
Rasool, S.T. et al. (2008) Immunol. Lett. 117:161.
Nold, M.F. et al. (2008) J. Immunol. 181:557.

Long Name

Interleukin 32

Entrez Gene IDs

9235 (Human)

Alternate Names

IL32; IL-32; IL-32alpha; IL-32beta; interleukin 32; interleukin-32 theta; interleukin-32; natural killer cell transcript 4; Natural killer cells protein 4; NK4; NK4IL-32delta; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; TAIFIL-32gamma; Tumor necrosis factor alpha-inducing factor

Product Datasheets

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Related Research Areas

Citations for Human IL-32 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Genetic variant in IL-32 is associated with the ex vivo cytokine production of anti-TNF treated PBMCs from rheumatoid arthritis patients
Authors: MSMA Damen, K Schraa, L Tweehuysen, AA den Broede, MG Netea, CD Popa, LAB Joosten
Sci Rep, 2018-09-19;8(1):14050.
Species: Human
Sample Types: Cell Culture Supernates
Inflammation-dependent secretion and splicing of IL-32{gamma} in rheumatoid arthritis.
Authors: Heinhuis B, Koenders MI, van de Loo FA, Netea MG, van den Berg WB, Joosten LA
Proc. Natl. Acad. Sci. U.S.A., 2011-03-07;108(12):4962-7.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Protection from RNA and DNA viruses by IL-32.
Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
J. Immunol., 2011-02-23;186(7):4110-8.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Electrochemiluminescent Assay
Interleukin-32 expression in the pancreas.
Authors: Nishida A, Andoh A, Inatomi O, Fujiyama Y
J. Biol. Chem., 2009-04-21;284(26):17868-76.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot