Human IL-36 alpha /IL-1F6 Antibody

Catalog #: AF1078
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1078
AF1078-SP

Detection of Human IL‑36 alpha /IL‑1F6 by Western Blot.

2 Images

Product Details

Citations (4)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-36 alpha /IL-1F6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑36 alpha /IL‑1F6 in direct ELISAs and Western blots. In Western blots, less than 2% cross‑reactivity with recombinant human (rh) rhIL-36 beta, rhIL-36 gamma, rhIL-1 alpha, rhIL-1 beta and rhIL-18 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IL‑36 alpha /IL‑1F6
Met1-Phe158
Accession # Q9UHA7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human IL‑36 alpha /IL‑1F6 by Western Blot. Western blot shows lysates of human tonsil tissue, HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-36a/IL-1F6. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-36a/IL-1F6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1078) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-36a/IL-1F6 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

View Larger

IL‑36 alpha /IL‑1F6 in Human Tonsil. IL-36a/IL-1F6 was detected in perfusion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-36a/IL-1F6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1078) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 alpha/IL-1F6

Human interleukin 1 family member #6 [IL-1F6; also Interleukin-36 alpha, IL36A, FIL-1 epsilon (epsilon)] is a member of the IL-1 family of proteins (1‑3). IL-1 family members include IL-1 beta, IL-1 alpha, IL‑1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F6 is synthesized as a 158 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (1‑3). It appears to be actively secreted (1). When found in cell lysate, it presents as an 18 kDa monomer (2). Human to mouse, full length IL-1F6 has 54% aa identity. Within the family, IL-1F6 is 30% aa identical to IL-1ra, and 27%, 31%, 36%, 46%, 57% and 28% aa identical to IL-1 beta, IL-1F5, F7, F8, F9 and F10, respectively. Cells reported to express IL-1F6 include monocytes, B cells and T cells (1, 4). Notably, IL-1F6 is the only novel IL-1 family member found to be expressed on T-cells. The receptor for IL-1F6 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL‑1F6, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NF-kappa B and MAPK in an IL‑1 Rrp2 dependent manner. This suggests that IL-1F6 may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

References

Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
Dunn, E. et al. (2001) Trends Immunol. 22:533.
Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Long Name

Interleukin 36 alpha/Interleukin 1 Family 6

Entrez Gene IDs

27179 (Human); 54448 (Mouse)

Alternate Names

FIL1 epsilon; FIL1; FIL1(EPSILON); FIL1E; IL-1 epsilon; IL1(EPSILON); IL1E; IL-1E; IL-1F6 (FIL-1-epsilon); IL1F6; IL-1F6; IL36 alpha; IL-36 alpha; IL36A; interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; Interleukin 36, Alpha; Interleukin-1 epsilon; interleukin-1 family member 6; Interleukin-36 Alpha

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

IL-1 Family

Citations for Human IL-36 alpha /IL-1F6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

A therapeutic strategy of parthenolide in improving imiquimod-induced psoriasis-like skin inflammation targeting IL-36/NETs through skin transdermal therapeutic system
Authors: Zhan, ZY;Zhang, ZH;Sun, RH;Wu, YL;Nan, JX;Lian, LH;
International immunopharmacology
Species: Human hepegivirus
Sample Types: Cell Lysates
Applications: Western Blot
Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells
Authors: JM Murrieta-C, RN Gutiérrez-, HM El-Shorafa, T Groten, S Rodríguez-, ME Cancino-Di, JC Cancino-Di, RR Favaro, UR Markert, DM Morales-Pr
International Journal of Molecular Sciences, 2020-12-30;22(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Interleukin-36 receptor mediates the crosstalk between plasma cells and synovial fibroblasts
Authors: V Schmitt, M Hahn, V Kästele, O Wagner, M Wiendl, A Derer, A Taddeo, S Hahne, A Radbruch, HM Jäck, W Schuh, D Mielenz, S Gay, G Schett, AJ Hueber, S Frey
Eur. J. Immunol., 2017-09-22;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression.
Authors: Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
J. Immunol., 2011-01-17;186(4):2613-22.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr